In vivo interactions of TTDA mutant proteins within TFIIH
| Autor: | Giuseppina Giglia-Mari, Stéphanie Cabantous, Julie Nonnekens, Pierre-Olivier Mari, Joris Slingerland |
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| Rok vydání: | 2013 |
| Předmět: |
Src Homology 2 Domain-Containing
Transforming Protein 1 Protein subunit Mutant Trichothiodystrophy Context (language use) Biology Transfection medicine.disease_cause Cell Line Protein–protein interaction chemistry.chemical_compound parasitic diseases medicine Humans Trichothiodystrophy Syndromes Protein Interaction Domains and Motifs Mutation DNA Cell Biology Fibroblasts medicine.disease Molecular biology Cell biology Protein Subunits Shc Signaling Adaptor Proteins chemistry Transcription factor II H Mutant Proteins Transcription Factor TFIIH DNA Damage Transcription Factors |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.126839 |
| Popis: | Trichothiodystrophy group A (TTD-A) patients carry a mutation in the transcription factor II H (TFIIH) subunit TTDA. Using a novel in vivo tripartite split-GFP system, we show that TTDA interacts with the TFIIH subunit p52 and the p52-TTDA-GFP product is incorporated into TFIIH. p52-TTDA-GFP is able to bind DNA and is recruited to UV-damaged DNA. Furthermore, we show that two patient mutated TTDA proteins can interact with p52, are able to bind to the DNA and can localize to damaged DNA. Our findings give new insights into the behavior of TTDA within the context of a living cell and thereby shed light on the complex phenotype of TTD-A patients. |
| Databáze: | OpenAIRE |
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