Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test
| Autor: | Gordon D. Harkiss, Neil J. Watt, Clare Whelan, Amanda O'Brien, John Clarke, Alastair Hayton |
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| Rok vydání: | 2017 |
| Předmět: |
Serum
0301 basic medicine Microbiology (medical) Tuberculosis 040301 veterinary sciences Clinical Biochemistry Immunology Paratuberculosis Serology 0403 veterinary science 03 medical and health sciences Antigen Diagnostic Laboratory Immunology Animals Immunology and Allergy Medicine Serologic Tests Immunoassay Mycobacterium bovis Goat Diseases biology business.industry Goats 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Antibodies Bacterial Milk 030104 developmental biology Herd biology.protein Antibody business Mycobacterium |
| Zdroj: | Clinical and Vaccine Immunology. 24 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00518-16 |
| Popis: | Tuberculosis in goats is usually diagnosed clinically, at postmortem, or by a positive skin test. However, none of these approaches detects all infected animals. Serology offers an additional tool to identify infected animals missed by current tests. We describe the use of the Enferplex Caprine TB serology test to aid the management of a large dairy goat herd undergoing a tuberculosis breakdown. Initial skin and serology testing showed that IgG antibodies were present in both serum and milk from 100% of skin test-positive animals and in serum and milk from 77.8 and 95.4% of skin test-negative animals, respectively. A good correlation was observed between serum and milk antibody levels. The herd had been vaccinated against Mycobacterium avium subsp. paratuberculosis , but no direct serological cross-reactions were found. Subsequent skin testing revealed 13.7% positive animals, 64.9% of which were antibody positive, while 42.1% of skin test-negative animals were seropositive. Antibody responses remained high 1 month later (57.1% positive), and the herd was slaughtered. Postmortem analysis of 20 skin test-negative goats revealed visible lesions in 6 animals, all of which had antibodies to six Mycobacterium bovis antigens. The results provide indirect evidence that serology testing with serum or milk could be a useful tool in the diagnosis and management of tuberculosis in goats. |
| Databáze: | OpenAIRE |
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