Methanolic extract of Ephedra alata ameliorates cisplatin-induced nephrotoxicity and hepatotoxicity through reducing oxidative stress and genotoxicity
| Autor: | Zaineb Dhaouefi, Imene Ben Toumia, Fairouz Sioud, Leila Chekir-Ghedira, Mouna Maatouk, Rihab Khlifi, Aida Lahmer, Kamel Ghedira |
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| Rok vydání: | 2019 |
| Předmět: |
DNA damage
Health Toxicology and Mutagenesis Ephedra 010501 environmental sciences Pharmacology medicine.disease_cause Kidney 01 natural sciences Antioxidants Nephrotoxicity Superoxide dismutase chemistry.chemical_compound Mice medicine Environmental Chemistry Animals 0105 earth and related environmental sciences biology Plant Extracts Methanol General Medicine Glutathione Malondialdehyde biology.organism_classification Pollution Oxidative Stress chemistry Catalase biology.protein Chemical and Drug Induced Liver Injury Cisplatin Ephedra alata Oxidative stress DNA Damage |
| Zdroj: | Environmental science and pollution research international. 27(11) |
| ISSN: | 1614-7499 |
| Popis: | Cisplatin (CP) is a powerful anticancer agent used in the treatment of a diverse type of cancers. Oxidative stress is one of the most important side effects limiting the use of cisplatin. The protective effects of methanolic extract (ME) and ephedrine (EP), major compound, of Ephedra alata on CP-induced damages were here assessed. Treatment with CP-induced nephrotoxicity and hepatotoxicity characterized by biochemical alterations. In fact, using CP reduced significantly glutathione (GSH) levels, enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), and increased malondialdehyde (MDA) content. Nonetheless, CP-treatment induced DNA damage at renal, hepatic, and blood cells and increased interferon gamma (IFNγ) level in serum. Co-treatments of mice with ME normalized relative kidney/body weight, restored biochemical and oxidative stress parameters, reduced DNA damage and IFNγ level. In conclusion, ME exhibited the best protective effect against CP damage compared with ephedrine. This is could be attributed to the presence of polysaccharides, organic acids, flavonoids, and tannins in addition to ephedrine alkaloids. These compounds were reported to play a major role in inhibiting and scavenging free radicals, providing an effective protection against CP- induced oxidative damage. Graphical abstract. |
| Databáze: | OpenAIRE |
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