Origins and unconventional behavior of neutrophils in developing zebrafish
| Autor: | Emma Colucci-Guyon, Karima Kissa, Hiroto Shinomiya, Elodie Mordelet, Philippe Herbomel, Agustín G. Zapata, Valérie Briolat, Emi Murayama, Michael J. Redd, Dorothée Le Guyader |
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| Přispěvatelé: | Macrophages et Développement de l'Immunité, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Huntsman Cancer Institute [Salt Lake City], University of Utah, Facultad de Ciencias Biológicas [Madrid], Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Graduate School of Medicine [Ehime, Japan], Ehime University [Matsuyama, Japon], This work was initially supported by an Avenir grant from Inserm (P.H.). D.L.G. was supported by a fellowship from Ministère de l'Education Nationale de la Recherche et de la Technologie., We thank Isabelle Godin, Véronique Witko-Sarsat, and Jean-Pierre Levraud for their critical reading of the manuscript, and J. M. Ghigo and W. Bitter for the GFP and DsRed expressing E coli bacteria, respectively., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Ehime University [Matsuyama] |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Embryo
Nonmammalian Neutrophils [SDV]Life Sciences [q-bio] MESH: Zebrafish / immunology MESH: Epidermal Cells MESH: Infections / immunology Biochemistry MESH: Neutrophils / cytology [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity MESH: Embryo Nonmammalian / cytology 0302 clinical medicine MESH: Animals Zebrafish MESH: Epidermis / embryology 0303 health sciences Phagocytes Microscopy Video biology MESH: Zebrafish / embryology MESH: Embryo Nonmammalian / embryology Embryo Hematology Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Epidermis / immunology Cell type Mesenchyme Immunology Danio Infections 03 medical and health sciences MESH: Phagocytes / immunology medicine Animals Cell Lineage Progenitor cell Yolk sac 030304 developmental biology MESH: Embryo Nonmammalian / immunology Macrophages MESH: Macrophages / cytology MESH: Neutrophils / immunology Cell Biology biology.organism_classification Embryonic stem cell MESH: Microscopy Video Epidermal Cells MESH: Phagocytes / cytology Epidermis MESH: Cell Lineage / immunology |
| Zdroj: | Blood Blood, 2008, 111 (1), pp.132-141. ⟨10.1182/blood-2007-06-095398⟩ Blood, American Society of Hematology, 2008, 111 (1), pp.132-141. ⟨10.1182/blood-2007-06-095398⟩ |
| ISSN: | 0006-4971 1528-0020 |
| DOI: | 10.1182/blood-2007-06-095398⟩ |
| Popis: | International audience; The first leukocytes that arise in the development of vertebrate embryos are the primitive macrophages, which differentiate in the yolk sac and then quickly invade embryonic tissues. These macrophages have been considered to constitute a separate lineage, giving rise to no other cell type. Using an in vivo photoactivatable cell tracer in the transparent zebrafish (Danio rerio) embryo, we demonstrated that this lineage also gave rise to an equal or higher number of neutrophilic granulocytes. We were surprised to find that the differentiation of these primitive neutrophils occurs only after primitive myeloid progenitors have dispersed in the tissues. By 2 days after fertilization, these neutrophils have become the major leukocyte type found wandering in the epidermis and mesenchyme. Like the primitive macrophages, all primitive and larval neutrophils express PU.1 and L-plastin and they are highly attracted to local infections, yet only a small fraction of them phagocytose microbes, and to a much lesser extent per cell than the macrophages. They are also attracted to variously stressed or malformed tissues, suggesting a wider role than antimicrobial defense. |
| Databáze: | OpenAIRE |
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