Effect of acute and long-term administration of gold nanoparticles on biochemical parameters in rat brain
| Autor: | Francieli Vuolo, Marcos Marques da Silva Paula, Gislaine T. Rezin, Felipe Dal-Pizzol, Eria Cardoso, Gabriel Alonso-Núñez, Gabriela K. Ferreira, Vanessa Moraes de Andrade, Cinara L. Gonçalves, R. Benavides, Monique Michels, Emilio L. Streck, Leticia S. Galant |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment Intraperitoneal injection Metal Nanoparticles Bioengineering 02 engineering and technology Pharmacology medicine.disease_cause Biomaterials 03 medical and health sciences medicine TBARS Animals Citrate synthase Rats Wistar biology Chemistry Neurotoxicity Brain 021001 nanoscience & nanotechnology medicine.disease Rats Oxidative Stress 030104 developmental biology Mitochondrial respiratory chain Biochemistry Mechanics of Materials Catalase biology.protein Creatine kinase Gold Energy Metabolism 0210 nano-technology Oxidative stress |
| Zdroj: | Materials Science and Engineering: C. 79:748-755 |
| ISSN: | 0928-4931 |
| Popis: | The present study investigated stress oxidative parameters and activities of enzymes of the energy metabolism in various brain structures. Rats were subjected to acute and long-term administration of gold nanoparticles (GNPs) with mean diameters of 10nm and 30nm. Adult (60days old) male Wistar rats received a single intraperitoneal injection (acute administration; 70μg·kg-1) or repeated injections once daily for 28days (long-term administration; 70μg·kg-1) of saline solution or GNPs (10nm or 30nm). Twenty-four hours after administration of the final dose, the animals were killed and the cerebral structures were isolated for enzyme analysis. In this study, we observed that the thiobarbituric acid-reactive species and carbonyl protein levels were decreased after acute administration of GNPs, whereas the superoxide dismutase activity was increased after acute and long-term of GNPs. The catalase activity was affected by the administration of GNPs. Furthermore, we have not found change in the citrate synthase activity. The succinate dehydrogenase, malate dehydrogenase, complexes I, II, II-III and IV, and creatine kinase activities were altered. These results indicate that inhibition energy metabolism can be caused by oxidative stress. |
| Databáze: | OpenAIRE |
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