Apparent inhibition of the plasma membrane Ca2+ pump by oleic acid
| Autor: | Frank F. Vincenzi, Pine Rw, Carrico Cj |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Erythrocytes
Time Factors ATPase Oleic Acids Calcium-Transporting ATPases In Vitro Techniques Critical Care and Intensive Care Medicine In vitro model chemistry.chemical_compound Medicine Ca2 pump Humans Magnesium biology Dose-Response Relationship Drug business.industry Vesicle Erythrocyte Membrane Biological Transport Acetylcholinesterase Oleic acid Membrane chemistry biology.protein Biophysics Surgery business |
| Zdroj: | The Journal of trauma. 23(5) |
| ISSN: | 0022-5282 |
| Popis: | The effects of oleic acid (oleate) were examined using two in vitro model systems. In a concentration-dependent fashion oleate activated or inhibited the (Ca2+ + Mg2+)-ATPase, or Ca2+ pump ATPase, of membranes isolated from human red blood cells (RBC's). Concentrations of oleate which inhibited the Ca2+ pump ATPase also inhibited the Na+-K+ pump ATPase. Likewise, in a concentration-dependent fashion oleate increased or abolished ATP dependent 45Ca2+ transport into inside-out vesicles (IOV's) prepared from human RBC's. Addition of 500 microM oleate to IOV's which had already accumulated 45Ca2+ resulted in rapid loss of the ion. The effect was shown to be due to membrane disruption; a conclusion based on oleate-induced unmasking of latent acetylcholinesterase activity in IOV preparations. The results are compatible with, but do not prove, that membrane disruption caused by circulating free fatty acids and similar membrane active agents might play a role in the cellular injury associated with certain pathophysiologic states. |
| Databáze: | OpenAIRE |
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