Oenothera rosea L´Hér. ex Ait attenuates acute colonic inflammation in TNBS-induced colitis model in rats: in vivo and in silico myeloperoxidase role
| Autor: | Martha Cecilia Rosales-Hernández, M. Jonathan Fragoso-Vázquez, María Estela Meléndez-Camargo, Alan R. Estrada-Pérez, Marlet Martínez-Archundia, Natalia Calva-Candelaria, Yazmín K. Márquez-Flores, Hortensia Montellano-Rosales, José Correa-Basurto |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Colon medicine.drug_class Interleukin-1beta Oenothera rosea Down-Regulation Pharmacology Nitric Oxide Inflammatory bowel disease Anti-inflammatory Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Animals Intestinal Mucosa Rats Wistar Colitis Peroxidase Inflammation biology Plant Extracts Chemistry General Medicine medicine.disease biology.organism_classification Rats Disease Models Animal 030104 developmental biology Oenothera Trinitrobenzenesulfonic Acid 030220 oncology & carcinogenesis Myeloperoxidase biology.protein Female Kaempferol |
| Zdroj: | Biomedicine & Pharmacotherapy. 108:852-864 |
| ISSN: | 0753-3322 |
| Popis: | Oenothera rosea L´Her. ex Ait is a species traditionally used in the treatment of inflammation, headache, stomach pain, infections, among others. The aim of this study was evaluating the acute anti-inflammatory activity of the aqueous extract of O. rosea by 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Rats were randomized into six groups: (I) Sham; (II) EtOH; (III) TNBS; and (IV–VI) 250, 500 and 750 mg/Kg, respectively. The colonic injury was induced (groups III–VI) by intrarectal instillation of 0.25 mL of TNBS (10 mg) in 50% ethanol. Groups I and II received an enema (0.25 mL) of physiological saline solution or 50% ethanol, respectively. Treatments were administered by oral gavage 48, 24 and 1 h prior, and 24 h after the induction. The inflammatory response was assessed considering the macroscopic and microscopic damage, the serum nitric oxide (NO), the colonic IL-1β levels, and the myeloperoxidase (MPO) activity. Moreover, we performed an LC–MS-based metabolite profiling, and a docking on the MPO. Doses of 500 and 750 mg/Kg showed a protective effect in the TNBS-induced colonic damage. This activity was related to the downregulation of evaluated parameters. Also, considering previous reports, 29 metabolites of 91 detected were selected for the docking, of which Isolimonic acid (29) and Kaempferol 3-(2′′,4′′-diacetylrhamnoside) (10) showed the highest affinity to MPO. The aqueous extract of O. rosea protected the TNBS-induced colonic damage in rats, an effect that could be associated with the presence of polyphenolic compounds, alkaloids, and terpenes; as well as their ability to down-regulate MPO activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect