The globin gene family of the cephalochordate amphioxus: implications for chordate globin evolution
| Autor: | Thorsten Burmester, Laurent Kiger, Thomas Hankeln, Serge N. Vinogradov, Georgia Panopoulou, Bettina Ebner, Michael C. Marden |
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| Přispěvatelé: | BMC, Ed., Institute of Molecular Genetics, Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft, Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Substitut du sang et pathologie moléculaire du globule rouge, Institut National de la Santé et de la Recherche Médicale (INSERM), Biocenter Grindel, Zoological Museum-University of Hamburg, Johannes Gutenberg - Universität Mainz (JGU) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
MESH: Sequence Analysis Protein MESH: Introns 2R hypothesis MESH: Amino Acid Sequence 0302 clinical medicine Chordata Nonvertebrate Sequence Analysis Protein Branchiostoma floridae hemic and lymphatic diseases MESH: Animals MESH: Phylogeny Phylogeny MESH: Evolution Molecular Genetics Cephalochordate 0303 health sciences [SDV.SA] Life Sciences [q-bio]/Agricultural sciences biology Cytoglobin Globins Multigene Family Research Article Genome evolution animal structures Evolution MESH: Bayes Theorem Molecular Sequence Data Chordate [SDV.BC]Life Sciences [q-bio]/Cellular Biology Evolution Molecular 03 medical and health sciences QH359-425 MESH: Globins Animals MESH: Chordata Nonvertebrate Amino Acid Sequence Globin [SDV.BC] Life Sciences [q-bio]/Cellular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology MESH: Molecular Sequence Data Bayes Theorem biology.organism_classification Introns Globin fold [SDE.BE] Environmental Sciences/Biodiversity and Ecology MESH: Multigene Family [SDE.BE]Environmental Sciences/Biodiversity and Ecology 030217 neurology & neurosurgery |
| Zdroj: | BMC Evolutionary Biology BMC Evolutionary Biology, BioMed Central, 2010, 10 (1), pp.370. ⟨10.1186/1471-2148-10-370⟩ BMC Evolutionary Biology, Vol 10, Iss 1, p 370 (2010) |
| ISSN: | 1471-2148 |
| DOI: | 10.1186/1471-2148-10-370⟩ |
| Popis: | Background The lancelet amphioxus (Cephalochordata) is a close relative of vertebrates and thus may enhance our understanding of vertebrate gene and genome evolution. In this context, the globins are one of the best studied models for gene family evolution. Previous biochemical studies have demonstrated the presence of an intracellular globin in notochord tissue and myotome of amphioxus, but the corresponding gene has not yet been identified. Genomic resources of Branchiostoma floridae now facilitate the identification, experimental confirmation and molecular evolutionary analysis of its globin gene repertoire. Results We show that B. floridae harbors at least fifteen paralogous globin genes, all of which reveal evidence of gene expression. The protein sequences of twelve globins display the conserved characteristics of a functional globin fold. In phylogenetic analyses, the amphioxus globin BflGb4 forms a common clade with vertebrate neuroglobins, indicating the presence of this nerve globin in cephalochordates. Orthology is corroborated by conserved syntenic linkage of BflGb4 and flanking genes. The kinetics of ligand binding of recombinantly expressed BflGb4 reveals that this globin is hexacoordinated with a high oxygen association rate, thus strongly resembling vertebrate neuroglobin. In addition, possible amphioxus orthologs of the vertebrate globin X lineage and of the myoglobin/cytoglobin/hemoglobin lineage can be identified, including one gene as a candidate for being expressed in notochord tissue. Genomic analyses identify conserved synteny between amphioxus globin-containing regions and the vertebrate β-globin locus, possibly arguing against a late transpositional origin of the β-globin cluster in vertebrates. Some amphioxus globin gene structures exhibit minisatellite-like tandem duplications of intron-exon boundaries ("mirages"), which may serve to explain the creation of novel intron positions within the globin genes. Conclusions The identification of putative orthologs of vertebrate globin variants in the B. floridae genome underlines the importance of cephalochordates for elucidating vertebrate genome evolution. The present study facilitates detailed functional studies of the amphioxus globins in order to trace conserved properties and specific adaptations of respiratory proteins at the base of chordate evolution. |
| Databáze: | OpenAIRE |
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