Effect of snuff on cytokeratin expression in oral vestibular sulcus epithelium
| Autor: | Marita Luomanen, Ilkka Heinaro, Risto-Pekka Happonen, Outi Tiitta, Kristiina Heikinheimo |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Tobacco Smokeless Keratosis Biopsy Stratified squamous epithelium Biology Epithelium Pathology and Forensic Medicine 03 medical and health sciences Basal (phylogenetics) Cytokeratin 0302 clinical medicine Keratin medicine Humans Fluorescent Dyes 030304 developmental biology chemistry.chemical_classification 0303 health sciences Hyperplasia Sulcular epithelium Mouth Mucosa Antibodies Monoclonal medicine.disease Plants Toxic medicine.anatomical_structure Gene Expression Regulation Otorhinolaryngology chemistry Fluorescent Antibody Technique Direct 030220 oncology & carcinogenesis Keratins Periodontics Leukoplakia Oral Oral Surgery Precancerous Conditions Biomarkers Fluorescein-5-isothiocyanate |
| Zdroj: | Journal of Oral Pathology and Medicine. 26:110-116 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/j.1600-0714.1997.tb00032.x |
| Popis: | Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral epithelium of the maxillary vestibular sulcus and clinically normal sulcular epithelium were studied by indirect immunofluorescence staining with a panel of monoclonal antibodies (MAbs). CK 14, a marker of stratified squamous epithelium was not seen expressed in 3/11 of the snuff user's specimens. Terminal differentiation markers, typical of cornified epithelia (CK 1, 9, 10 and 11), were detected suprabasally in the snuff user's keratosis but not in the normal control epithelium. The use of snuff seemed to change the CK staining pattern of the mucosa so that it resembles more that of a cornified type of epithelium. Simple epithelial-type CK were included in the study in order to establish the CK profile of the snuff-induced keratosis, for comparison with normal and dysplastic lesions. MAb to CK 7 and 19 showed reactivity in the basal cells and suprabasally whereas the monospecific MAb anti-CK 7 showed suprabasal staining both in the control and affected epithelia. By using MAbs, we found no immunoreactivity against CK 18 either in normal or affected epithelia, whereas we found suprabasal reaction (5/11) against CK 8 in the snuff user's epithelia. The two MAbs demonstrating the expression of CK 19, normally confined to the basal cells of the stratified squamous epithelium, showed variable patterns of expression both in basal cells and suprabasally in the snuff lesions. The results show that use of oral snuff causes some alterations in the CK expression pattern of the affected epithelium. Whether the alterations are indicative of a premalignant change is, however, uncertain. The results encourage further studies on the subject. |
| Databáze: | OpenAIRE |
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