Regulatory role of hippocampal PI3K and mTOR signaling pathway in NMDA-induced infant spasm rats
| Autor: | Yan Meng, Lin Wan, Guang Yang, Xiu-Yu Shi, Wei-Hua Ren, Li-Ping Zou, Jing Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Spasm N-Methylaspartate P70-S6 Kinase 1 Hippocampal formation Pharmacology Hippocampus Epileptogenesis Rats Sprague-Dawley Wortmannin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Phosphorylation Protein Kinase Inhibitors Protein kinase B PI3K/AKT/mTOR pathway Sirolimus Cell Death TOR Serine-Threonine Kinases Neurogenesis General Medicine Disease Models Animal 030104 developmental biology Neurology chemistry Female Neurology (clinical) Phosphatidylinositol 3-Kinase 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Neurological Research. 41:1075-1082 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1080/01616412.2019.1672412 |
| Popis: | Objectives: To explore the role of mTOR signaling pathway in modulating epileptogenesis in an N-methyl-D-aspartic acid (NMDA)-induced infant spasm (IS) rat model. Methods: After inducing IS successfully, the phosphorylation status of PI3K, Akt, mTOR and S6K of brain and hippocampus tissues was assessed using western blot and immunochemistry analysis, respectively. The possible mechanism of mTOR signaling pathway was evaluated by the, inhibitors for mTOR and PI3K, rapamycin and wortmannin, respectively. The inhibitors were injected into the intraperitoneal space of the rats to examine the effects of PI3K and mTOR in IS rat model. Results: The phosphorylated levels of mTOR and PI3K in hippocampus increased significantly (P < 0.05) 7 days after IS induction in rats. After administration of wortmannin, the phosphorylated levels of PI3K and mTOR decreased. However, only the phosphorylated level of mTOR decreased obviously after rapamycin administration. No obvious neurogenesis was found after IS induction. Discussion: Results of the present study suggest that hippocampal PI3K may be another potential target for IS treatment. |
| Databáze: | OpenAIRE |
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