Molecular characterization, expression and methylation status analysis of BMP4 gene in skin tissue of Liaoning cashmere goat during hair follicle cycle
| Autor: | Shu H Yang, Ze Y Wang, Yun L Dang, Hui L. Xue, Wen L. Bai, Yu B. Zhu, Shi Q. Wang, Liang Deng, Rong H. Yin, Jiao J. Wang, Yu Y. Cong, Dan Guo |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Models Molecular animal structures DNA Complementary Transcription Genetic Protein Conformation Plant Science Bone Morphogenetic Protein 4 Biology 03 medical and health sciences 0302 clinical medicine Complementary DNA Genetics medicine Cashmere goat Animals Protein Interaction Domains and Motifs Amino Acid Sequence Cloning Molecular Gene Skin Messenger RNA integumentary system Base Sequence Goats General Medicine Methylation Sequence Analysis DNA DNA Methylation Hair follicle Cell biology Open reading frame 030104 developmental biology medicine.anatomical_structure Bone morphogenetic protein 4 Gene Expression Regulation 030220 oncology & carcinogenesis Insect Science embryonic structures Animal Science and Zoology CpG Islands Hair Follicle |
| Zdroj: | Genetica. 144(4) |
| ISSN: | 1573-6857 |
| Popis: | Bone morphogenetic protein 4 (BMP4) is a member of the bone morphogenetic protein family (BMPs). It is involved in the development and cycle of hair follicle, as well as, is thought to be a potential candidate gene for cashmere traits in goats. In the present study, we isolated and characterized a full-length open reading frame (ORF) of BMP4 cDNA from the skin tissue of Liaoning cashmere goat, and investigated the transcriptional pattern and methylation status of BMP4 gene in skin tissue of this breed during different stages of hair follicle cycle. The sequence analysis indicated that the isolated cDNA was 1264-bp in length containing a complete ORF of 1230-bp. It encoded a precursor peptide of 409 amino acids with a signal peptide of 19 amino acids. The structural analysis indicated that goat BMP4 contains typical TGF-β propeptide and TGF-β domains. In skin tissue, BMP4 is generally transcribed in an ascendant pattern from anagen to telogen. The methylation level of 5′ flanking regulatory region of BMP4 gene might be involved in its mRNA expression in skin tissue: a higher BMP4 methylation level in skin coincides with a lower expression of BMP4 mRNA. These results from the present work provided a foundation for further insight into the functional and regulatory characteristics of BMP4 in the development and cycle of hair follicle in Liaoning Cashmere goat. |
| Databáze: | OpenAIRE |
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