Regulation of Alanine Dehydrogenase in Bacillus licheniformis
| Autor: | S. M. McCowen, P V Phibbs |
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| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Alanine dehydrogenase Malates Bacillus Dehydrogenase Nicotinamide adenine dinucleotide Microbiology Ammonium Chloride Chromatography DEAE-Cellulose chemistry.chemical_compound Glutamates Oxidoreductase Chemical Precipitation Bacillus licheniformis Pyruvates Molecular Biology Spores Bacterial chemistry.chemical_classification Alanine Cell-Free System biology Stereoisomerism Metabolism NAD biology.organism_classification Enzyme assay Glucose Biochemistry chemistry Ammonium Sulfate Spectrophotometry Enzymology biology.protein Amino Acid Oxidoreductases NAD+ kinase NADP |
| Zdroj: | Journal of Bacteriology. 118:590-597 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.118.2.590-597.1974 |
| Popis: | Cell extracts of Bacillus licheniformis were found to contain nicotinamide adenine dinucleotide (NAD)-dependent l -alanine dehydrogenase (ADH) ( l -alanine: NAD oxidoreductase, EC 1.4.1.1). High specific activities (3.5 to 6.0 IU/mg of protein) were found in extracts of cells throughout growth cycles only when l -alanine served as the primary source of carbon or carbon and nitrogen. Specific activities were minimal (0.02 to 0.04 IU/mg of protein) during growth on glucose, but increased at least sevenfold during the first 5 h of postlogarithmic-phase metabolism. Addition of 10 mM glucose to cultures during logarithmic-phase growth on l -alanine resulted in a rapid decrease in enzyme activity. Addition of 20 mM l -alanine to cells near the completion of log-phase growth on glucose resulted in a 20-fold increase in ADH specific activity during less than one cell generation. Extracts of postlogarithmic-phase cells cultured on glucose, malate, l -glutamate, or Casamino Acids contained intermediate levels of ADH activity. The enzyme was partially purified from crude extracts of B. licheniformis , and apparent kinetic constants were estimated. A role for ADH in the catabolism of l -alanine to pyruvate during vegetative growth on l -alanine and during sporulation of cells cultured on glucose is proposed on the basis of these experimental results. |
| Databáze: | OpenAIRE |
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