Inflammatory response in rat lungs with recurrent exposure to welding fumes
| Autor: | Se-Myo Park, Mi-Jin Yang, Seokjoo Yoon, Myung-Sang Kwon, Jung-Hwa Oh, Chang-Woo Song, Il Je Yu, Jeong-Doo Heo, Han-Jin Park, Young-Su Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Pathology medicine.medical_specialty Health Toxicology and Mutagenesis Blotting Western Inflammation Air Pollutants Occupational Lung injury Toxicology Rats Sprague-Dawley Transcriptome Gene expression medicine Animals Cluster Analysis Welding Lung Analysis of Variance Inhalation Exposure business.industry Gene Expression Profiling Pneumoconiosis technology industry and agriculture Public Health Environmental and Occupational Health Lung Injury respiratory system medicine.disease Immunohistochemistry Rats Up-Regulation respiratory tract diseases Gene expression profiling medicine.anatomical_structure medicine.symptom business Bronchoalveolar Lavage Fluid Signal Transduction |
| Zdroj: | Toxicology and Industrial Health. 28:203-215 |
| ISSN: | 1477-0393 0748-2337 |
| DOI: | 10.1177/0748233711410906 |
| Popis: | As chronic exposure to welding fumes causes pulmonary diseases, such as pneumoconiosis, public concern has increased regarding continued exposure to these hazardous gases in the workplace. In a previous study, the inflammatory response to welding fume exposure was analysed in rat lungs in the case of recurrent exposure and recovery periods. Thus using lung samples, well-annotated by histological observation and biochemical analysis, this study examines the gene expression profiles to identify phenotype-anchored genes corresponding to lung inflammation and the repair phenomenon after recurrent welding fume exposure. Seven genes ( Mmp12, Cd5l, LOC50101, LOC69183, Spp1, and Slc26a4) were found to be significantly up-regulated according to the severity of the lung injury. In addition, the transcription and translation of Trem2, which was up-regulated in response to the repair process, were validated using a real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The differentially expressed genes in the exposure and recovery groups were also classified using k-means and hierarchical clustering, plus their toxicological function and canonical pathways were further analysed using Ingenuity Pathways Analysis Software. As a result, this comprehensive and integrative analysis of the transcriptional changes that occur during repeated exposure provides important information on the inflammation and repair processes after welding-fume-induced lung injury. |
| Databáze: | OpenAIRE |
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