Tumor growth suppression by implantation of an anti-CD25 antibody-immobilized material near the tumor via regulatory T cell capture
| Autor: | Rino Tokunaga, Naoko Nakamura, Tsuyoshi Kimura, Akio Kishida, Yoshihide Hashimoto |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Regulatory T cell
mouse model chemical and pharmacologic phenomena regulatory t cell regulatory t cell capture medicine cancer General Materials Science Tumor growth word IL-2 receptor Materials of engineering and construction. Mechanics of materials biology 30 Bio-inspired and biomedical materials 212 Surface and interfaces 211 Scaffold / Tissue engineering/Drug delivery Chemistry Cancer anti-cd25 antibody hemic and immune systems medicine.disease Focus on Trends in Biomaterials in Japan medicine.anatomical_structure TA401-492 Cancer research biology.protein Antibody TP248.13-248.65 Research Article Biotechnology |
| Zdroj: | Science and Technology of Advanced Materials, Vol 22, Iss 1, Pp 607-615 (2021) Science and Technology of Advanced Materials article-version (VoR) Version of Record |
| ISSN: | 1878-5514 1468-6996 |
| DOI: | 10.1080/14686996.2021.1944782 |
| Popis: | In this study, we designed and synthesized an implantable anti-CD25 antibody-immobilized polyethylene (CD25-PE) mesh to suppress tumor growth by removing regulatory T cells (Tregs). The PE mesh was graft-polymerized with poly(acrylic acid), and the anti-mouse CD25 antibody was then immobilized using the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide reaction. Immobilization of the antibody on the PE mesh was confirmed by immunostaining. The CD25-PE mesh could effectively and selectively capture CD25-positive cells through antigen-antibody interactions when the CD25-PE mesh was incubated with a suspension of mouse spleen cells, including CD25-positive cells. In addition, implantation of the CD25-PE mesh into mice subcutaneously demonstrated the Treg-capturing ability of the CD25-PE mesh with only a weak inflammatory reaction. In tumor-bearing mice, tumor growth was suppressed by subcutaneous implantation of the CD25-PE mesh near the tumor for 1 week. These results suggested that the anti-CD25 antibody-immobilized material could capture Tregs in vivo and inhibit tumor proliferation in a limited tumor-bearing mouse model. Further research is needed to facilitate cancer immunotherapy using implantable anti-CD25 antibody-immobilized material as a Treg-capturing device. Graphical abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|