Bisphenol-A affects male fertility via fertility-related proteins in spermatozoa
| Autor: | Myung-Geol Pang, Buom-Yong Ryu, Saidur Rahman, Woo-Sung Kwon, June-Sub Lee, Sung-Jae Yoon |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty endocrine system Acrosome reaction Embryonic Development Biology Endocrine Disruptors Article Andrology chemistry.chemical_compound Mice Human fertilization Adenosine Triphosphate Phenols Internal medicine Protein Interaction Mapping medicine Animals Protein Interaction Maps Benzhydryl Compounds Phosphorylation Protein kinase A Sperm motility reproductive and urinary physiology Multidisciplinary urogenital system Embryogenesis Sperm Cyclic AMP-Dependent Protein Kinases Spermatozoa Xenoestrogen Endocrinology Fertility chemistry Fertilization Sperm Motility Reactive Oxygen Species Sperm Capacitation hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Scientific Reports SCIENTIFIC REPORTS(5) |
| ISSN: | 2045-2322 |
| Popis: | The xenoestrogen bisphenol-A (BPA) is a widespread environmental contaminant that has been studied for its impact on male fertility in several species of animals and humans. Growing evidence suggests that xenoestrogens can bind to receptors on spermatozoa and thus alter sperm function. The objective of the study was to investigate the effects of varying concentrations of BPA (0.0001, 0.01, 1 and 100 μM for 6 h) on sperm function, fertilization, embryonic development and on selected fertility-related proteins in spermatozoa. Our results showed that high concentrations of BPA inhibited sperm motility and motion kinematics by significantly decreasing ATP levels in spermatozoa. High BPA concentrations also increased the phosphorylation of tyrosine residues on sperm proteins involved in protein kinase A-dependent regulation and induced a precocious acrosome reaction, which resulted in poor fertilization and compromised embryonic development. In addition, BPA induced the down-regulation of β-actin and up-regulated peroxiredoxin-5, glutathione peroxidase 4, glyceraldehyde-3-phosphate dehydrogenase and succinate dehydrogenase. Our results suggest that high concentrations of BPA alter sperm function, fertilization and embryonic development via regulation and/or phosphorylation of fertility-related proteins in spermatozoa. We conclude that BPA-induced changes in fertility-related protein levels in spermatozoa may be provided a potential cue of BPA-mediated disease conditions. |
| Databáze: | OpenAIRE |
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