Improved assay and mechanism of the reaction catalyzed by the chitin synthase from Saccharomyces cerevisiae
| Autor: | Marianne Fähnrich, Jan Ahlers |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
chemistry.chemical_classification
Chitin Synthase Uridine Diphosphate N-Acetylglucosamine biology Mechanism (biology) Saccharomyces cerevisiae Cell Membrane Substrate (chemistry) Chitin synthase biology.organism_classification Biochemistry Binding Competitive Catalysis Acetylglucosamine Kinetics Enzyme chemistry Product inhibition Glucosyltransferases biology.protein Filtration Initial rate |
| Zdroj: | European journal of biochemistry. 121(1) |
| ISSN: | 0014-2956 |
| Popis: | 1 An improved filtration method is introduced to perform kinetic investigations on the chitin synthase reaction. This method is especially suited for the assay of a large number of samples necessary in enzyme kinetic studies. 2 From initial rate data the possibility could be excluded that the two-substrate reactions occurs by a random or a ping-pong mechanism. 3 Investigations of product inhibition exclude a rapid random mechanism but favour an ordered mechanism with UDP-N-acetylglucosamine as the first substrate. 4 This result was confirmed by isotope exchange studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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