Multi-walled carbon nanotube oxidation dependent keratinocyte cytotoxicity and skin inflammation
| Autor: | Lisa A. DeLouise, Sarah J. Phelan-Dickenson, Brian C. Palmer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
Allergy Cell Survival Health Toxicology and Mutagenesis lcsh:Industrial hygiene. Industrial welfare Carboxylic Acids Dermatitis Carbon nanotube Toxicology Basophil degranulation Dermatitis Contact Cell Degranulation law.invention Cell Line 03 medical and health sciences Nanoparticle law lcsh:RA1190-1270 medicine Animals Edema Humans Cytotoxicity lcsh:Toxicology. Poisons Skin chemistry.chemical_classification 0303 health sciences Reactive oxygen species Mice Hairless Chemistry Nanotubes Carbon Research 030311 toxicology General Medicine Mast cell 3. Good health Mice Inbred C57BL HaCaT medicine.anatomical_structure Carboxylation Neutrophil Infiltration Biophysics Cytokines Dinitrofluorobenzene Keratinocyte Oxidation-Reduction lcsh:HD7260-7780.8 |
| Zdroj: | Particle and Fibre Toxicology Particle and Fibre Toxicology, Vol 16, Iss 1, Pp 1-15 (2019) |
| ISSN: | 1743-8977 |
| Popis: | Background The effects of carbon nanotubes on skin toxicity have not been extensively studied; however, our lab has previously shown that a carboxylated multi-walled carbon nanotube (MWCNT) exacerbates the 2, 4-dinitrofluorobenzene induced contact hypersensitivity response in mice. Here we examine the role of carboxylation in MWCNT skin toxicity. Results MWCNTs were analyzed by transmission electron microscopy, zetasizer, and x-ray photoelectron spectroscopy to fully characterize the physical properties. Two MWCNTs with different levels of surface carboxylation were chosen for further testing. The MWCNTs with a high level of carboxylation displayed increased cytotoxicity in a HaCaT keratinocyte cell line, compared to the MWCNTs with intermediate levels of carboxylation. However, neither functionalized MWCNT increased the level of in vitro reactive oxygen species suggesting an alternative mechanism of cytotoxicity. Each MWCNT was tested in the contact hypersensitivity model, and only the MWCNTs with greater than 20% surface carboxylation exacerbated the ear swelling responses. Analysis of the skin after MWCNT exposure reveals that the same MWCNTs with a high level of carboxylation increase epidermal thickness, mast cell and basophil degranulation, and lead to increases in polymorphonuclear cell recruitment when co-administered with 2, 4-dinitrofluorobenzene. Conclusions The data presented here suggest that acute, topical application of low doses of MWCNTs can induce keratinocyte cytotoxicity and exacerbation of allergic skin conditions in a carboxylation dependent manner. Electronic supplementary material The online version of this article (10.1186/s12989-018-0285-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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