An Intermediate Concentration of Calcium with Antioxidant Supplement in Culture Medium Enhances Proliferation and Decreases the Aging of Bone Marrow Mesenchymal Stem Cells
| Autor: | Je Ken Chang, Hsuan-Ti Huang, Lin Kang, Tsung Lin Cheng, Shu-Chun Chuang, Cheng Jung Ho, Sung Yen Lin, Mei-Ling Ho, Mon Juan Lee, Yi Shan Lin, Chung-Hwan Chen, Hui Ting Chen, Chung Da Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Telomerase
culture medium senescence medicine.medical_treatment Cellular differentiation Cell Separation Antioxidants Article Catalysis Inorganic Chemistry lcsh:Chemistry Tissue engineering Antigens CD medicine Humans Cell Lineage Physical and Theoretical Chemistry Cell Shape Molecular Biology lcsh:QH301-705.5 Cells Cultured Cellular Senescence Spectroscopy Osteoblasts cell aging Chemistry Growth factor Organic Chemistry Telomere Homeostasis Mesenchymal Stem Cells General Medicine Culture Media Computer Science Applications Cell biology Telomere cell differentiation medicine.anatomical_structure cell proliferation lcsh:Biology (General) lcsh:QD1-999 Calcium Bone marrow Stem cell Tumor Suppressor p53-Binding Protein 1 Cell aging Biomarkers DNA Damage |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2095, p 2095 (2021) International Journal of Molecular Sciences Volume 22 Issue 4 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Human bone marrow stem cells (HBMSCs) are isolated from the bone marrow. Stem cells can self-renew and differentiate into various types of cells. They are able to regenerate kinds of tissue that are potentially used for tissue engineering. To maintain and expand these cells under culture conditions is difficult—they are easily triggered for differentiation or death. In this study, we describe a new culture formula to culture isolated HBMSCs. This new formula was modified from NCDB 153, a medium with low calcium, supplied with 5% FBS, extra growth factor added to it, and supplemented with N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate to maintain the cells in a steady stage. The cells retain these characteristics as primarily isolated HBMSCs. Moreover, our new formula keeps HBMSCs with high proliferation rate and multiple linage differentiation ability, such as osteoblastogenesis, chondrogenesis, and adipogenesis. It also retains HBMSCs with stable chromosome, DNA, telomere length, and telomerase activity, even after long-term culture. Senescence can be minimized under this new formulation and carcinogenesis of stem cells can also be prevented. These modifications greatly enhance the survival rate, growth rate, and basal characteristics of isolated HBMSCs, which will be very helpful in stem cell research. |
| Databáze: | OpenAIRE |
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