Cording mycobacterium tuberculosis bacilli have a key role in the progression towards active tuberculosis, which is stopped by previous immune response
| Autor: | Clara Prats, Martí Català, Esther Julián, Pere-Joan Cardona, P. J. Cardona, Lilibeth Arias, Cristina Vilaplana, Víctor Campo-Pérez |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Doctorat en Física Computacional i Aplicada, Universitat Politècnica de Catalunya. Departament de Física, Universitat Politècnica de Catalunya. BIOCOM-SC - Grup de Biologia Computacional i Sistemes Complexos |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Chemokine neutrophilic extracellular traps Neutrophils medicine.medical_treatment 030106 microbiology Cording Microbiology Virulence factor Mycobacterium tuberculosis 03 medical and health sciences Immune system neutrophils In vivo Virology Extracellular Medicine BCG cxcl-1 C3HeB/FeJ mice lcsh:QH301-705.5 c3heb/fej mice cording biology Matemàtiques i estadística::Anàlisi numèrica::Modelització matemàtica [Àrees temàtiques de la UPC] business.industry CXCL-1 mycobacterium tuberculosis Tuberculosi -- Epidemiologia biology.organism_classification 3. Good health Vaccination 030104 developmental biology Cytokine lcsh:Biology (General) biology.protein bcg Neutrophilic extracellular traps business |
| Zdroj: | Microorganisms, Vol 8, Iss 2, p 228 (2020) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Microorganisms Volume 8 Issue 2 |
| Popis: | Cording was the first virulence factor identified in Mycobacterium tuberculosis (Mtb). We aimed to ascertain its role in the induction of active tuberculosis (TB) in the mouse strain C3HeB/FeJ by testing the immunopathogenic capacity of the H37Rv strain. We have obtained two batches of the same strain by stopping their growth in Proskauer Beck liquid medium once the mid-log phase was reached, in the noncording Mtb (NCMtb) batch, and two days later in the cording Mtb (CMtb) batch, when cording could be detected by microscopic analysis. Mice were challenged with each batch intravenously and followed-up for 24 days. CMtb caused a significant increase in the bacillary load at an early stage post-challenge (day 17), when a granulomatous response started, generating exudative lesions characterized by neutrophilic infiltration, which promoted extracellular bacillary growth together with cording formation, as shown for the first time in vivo. In contrast, NCMtb experienced slight or no bacillary growth and lesions could barely be detected. Previous Bacillus Calmette-Gué rin (BCG) vaccination or low dose aerosol (LDA) Mtb infection were able to delay the progression towards active TB after CMtb challenge. While BCG vaccination also reduced bacillary load when NCMtb was challenged, LDA did not, and its proliferative lesions experienced neutrophil infiltration. Analysis of lung cytokine and chemokine profiles points to their capacity to block the production of CXCL-1 and further amplification of IL-1&beta IL-17 and neutrophilic extracellular trap formation, all of which are essential for TB progression. These data highlight the key role of cording formation in the induction of active TB. |
| Databáze: | OpenAIRE |
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