Inter-Individual Differences in RNA Levels in Human Peripheral Blood
| Autor: | Hamid R. Eghbalnia, Piotr Chomczynski, Karol Mackey, Michal Rymaszewski, William W. Wilfinger, Amy Kennedy |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Physiology Molecular biology Cell Gene Expression lcsh:Medicine Biochemistry Polymerase Chain Reaction Sequencing techniques Gene expression Medicine and Health Sciences Small nucleolar RNAs RNA structure lcsh:Science Aged 80 and over Genes Essential Multidisciplinary RNA sequencing Hematology Middle Aged Body Fluids Nucleic acids RNA isolation Blood medicine.anatomical_structure Ribosomal RNA Female RNA extraction Anatomy Research Article Cell biology Cellular structures and organelles Biology Biomolecular isolation 03 medical and health sciences Extraction techniques Genetics medicine Humans RNA folding RNA Messenger Non-coding RNA Aged Messenger RNA Significant difference lcsh:R Biology and Life Sciences Genetic Variation Reproducibility of Results RNA DNA Peripheral blood Gene regulation Blood Cell Count Research and analysis methods Macromolecular structure analysis Molecular biology techniques 030104 developmental biology Immunology lcsh:Q Ribosomes |
| Zdroj: | PLoS ONE, Vol 11, Iss 2, p e0148260 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging in age from 50 to 89 years. In this group, the average total RNA level was 14.59 μg/ml of blood, with no statistically significant difference between females and males. The individual RNA level ranged from 6.7 to 22.7 μg/ml of blood. In healthy subjects, the repeated sampling of an individual's blood showed that RNA level, whether high or low, was stable. The inter-individual differences in RNA level in blood can be attributed to both, differences in cell number and the amount of RNA per cell. The 3.4-fold range of inter-individual differences in total RNA levels, documented herein, should be taken into account when evaluating the results of quantitative RT-PCR and/or RNA sequencing studies of human blood. Based on the presented results, a comprehensive assessment of gene expression in blood should involve determination of both the amount of mRNA per unit of total RNA (U / ng RNA) and the amount of mRNA per unit of blood (U / ml blood) to assure a thorough interpretation of physiological or pathological relevance of study results. |
| Databáze: | OpenAIRE |
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