Screening Platform for Cell Contact Guidance Based on Inorganic Biomaterial Micro/nanotopographical Gradients
| Autor: | Qihui Zhou, Carlos F. Guimarães, Patrick van Rijn, Theo G. van Kooten, Olga E. Castañeda Ocampo, Philipp T. Kühn |
|---|---|
| Přispěvatelé: | Polymer Chemistry and Bioengineering, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Nanotechnology and Biophysics in Medicine (NANOBIOMED) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biomimetic surface stem cell behavior OSTEOGENIC DIFFERENTIATION Materials science Surface Properties BONE-MARROW Biocompatible Materials Nanotechnology 02 engineering and technology Matrix (biology) high-throughput screening MESENCHYMAL STEM-CELLS Focal adhesion 03 medical and health sciences Cell Adhesion Humans General Materials Science Nanotopography Cytoskeleton Cell adhesion Cell contact NANOPHASE CERAMICS FOCAL ADHESIONS Biomaterial Mesenchymal Stem Cells IN-VITRO NANOTOPOGRAPHY Silicon Dioxide 021001 nanoscience & nanotechnology OSTEOBLAST ADHESION Nanostructures SURFACE-TOPOGRAPHY 030104 developmental biology inorganic biomaterials 0210 nano-technology Filopodia topographical gradient MATRIX Research Article |
| Zdroj: | ACS Applied Materials & Interfaces, 9(37), 31433-31445. AMER CHEMICAL SOC ACS Applied Materials & Interfaces |
| ISSN: | 1944-8244 |
| Popis: | High -throughput screening (HTS) methods based on topography gradients or arrays have been extensively used to investigate cell material interactions. However, it is a huge technological challenge to cost efficiently prepare topographical gradients of inorganic biomaterials due to their inherent material properties. Here, we developed a novel strategy translating PDMS-based wrinkled topography gradients with amplitudes from 49 to 2561 nm and wavelengths between 464 and 7121 nm to inorganic biomaterials (Sio(2), Ti/Tio(2), Cr/Cro(3), and AL(2)O(3)) which are frequently used clinical materials. Optimal substratum conditions promoted human bone-marrow derived mesenchymal stem cell alignment, elongation, cytoskeleton arrangement, filopodia development as well as cell adhesion in vitro, which depended both on topography and interface material. This study displays a positive correlation between cell alignment and the orientation of cytoskeleton, filopodia, and focal adhesions. This platform vastly minimizes the experimental efforts both for inorganic material interface engineering and cell biological assessments in a facile and effective approach. The practical application of the HTS technology is expected to aid in the acceleration of developments of inorganic clinical biomaterials. |
| Databáze: | OpenAIRE |
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