Analysis of glycogen metabolic pathway utilization by dendritic cells and T cells using custom phenotype metabolic assays
| Autor: | Eyal Amiel, Phyu M. Thwe |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Indoles T-Lymphocytes Immunology Cell Cell Culture Techniques Biology Article Cell Line 03 medical and health sciences Mice Immune system medicine Immunology and Allergy Animals Humans Coloring Agents Effector Glycogen Phosphorylase Phenotype microarray Metabolism Dendritic Cells Microarray Analysis Phenotype Amides Cell biology Metabolic pathway 030104 developmental biology medicine.anatomical_structure Biological Assay Energy Metabolism Oxidation-Reduction Function (biology) Glycogen Metabolic Networks and Pathways |
| Zdroj: | Journal of immunological methods. 458 |
| ISSN: | 1872-7905 |
| Popis: | In the field of immunology, there is an increasing interest in cellular energy metabolism and its outcome on immune cell effector function. Activation of immune cells leads to rapid metabolic changes that are central to cellular biology in order to support the effector responses. Therefore, the need for user-friendly and dependable assay technologies to address metabolic regulation and nutrient utilization in immune cells is an important need in this field. Redox-dye reduction-based Phenotype MicroArray (PM) assays, which measure NADH reduction as a readout, developed by Biolog Inc., provide a wide screening of metabolites both in bacteria and mammalian cells. In this study, we delineate a detailed protocol of a customized Biolog assay for investigation of a specific metabolic pathway of interest. The option to be able to easily customize this technology offers researchers with a convenient assay platform to methodically examine specific nutrient substrates or metabolic pathways of interest in a rapid and cost effective manner. |
| Databáze: | OpenAIRE |
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