Methods of preparation of recombinant cytokine proteins
| Autor: | S.A. Yakimov, L. N. Shingarova, A. N. Wulfson, V.E. Klyushnichenko, Korobko Vyacheslav G, R. V. Tikhonov, Pechenov Se |
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| Rok vydání: | 1998 |
| Předmět: |
Free-flow electrophoresis
Electrophoresis Chromatography Chemistry Tumor Necrosis Factor-alpha Size-exclusion chromatography medicine.disease_cause High-performance liquid chromatography Recombinant Proteins law.invention Recombinant tumor necrosis factor Capillary electrophoresis law Mutation medicine Recombinant DNA Escherichia coli Cytokines Humans Centrifugation Sequence Analysis Biotechnology |
| Zdroj: | Protein expression and purification. 14(2) |
| ISSN: | 1046-5928 |
| Popis: | Two schemes for efficient and productive isolation for mutant human recombinant tumor necrosis factor-α (TNF-α R32H) from Escherichia coli cells were developed. The methods include membrane filtration, ion-exchange chromatography and gel filtration, and centrifugation with subsequent free-flow electrophoresis as an alternative procedure. The target product was obtained as active trimer with total yield more than 50% and greater than 98% purity according to PAGE, size-exclusion chromatography, HPLC, and HPCE. |
| Databáze: | OpenAIRE |
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