Development of an ELISA using a recombinant 41 kDa partial protein (P45N′) for the detection of Riemerella anatipestifer infections in ducks
| Autor: | Hilda Loh, Hai-Meng Tan, Kim Lee Chua, Jimmy Kwang, Joachim Frey, Bin Huang |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Serotype
Salmonella animal diseases Blotting Western Molecular Sequence Data Enzyme-Linked Immunosorbent Assay medicine.disease_cause Flavobacterium Polymerase Chain Reaction Sensitivity and Specificity Microbiology Sepsis Escherichia coli medicine Animals Yersinia enterocolitica Poultry Diseases Base Sequence General Veterinary biology Riemerella anatipestifer Sequence Analysis DNA General Medicine biology.organism_classification Antibodies Bacterial Virology Enterobacteriaceae Recombinant Proteins Specific Pathogen-Free Organisms Citrobacter freundii Aeromonas hydrophila Ducks Gram-Negative Bacterial Infections Bacterial Outer Membrane Proteins |
| Zdroj: | Veterinary Microbiology. 88:339-349 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(02)00123-2 |
| Popis: | Riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. An indirect enzyme-linked immunosorbent assay (ELISA) has been developed to facilitate early detection of R. anatipestifer infection in ducks. The antigen used was a recombinant 41 kDa N-terminal fragment (rP45N') of a newly characterized R. anatipestifer potential surface protein, P45, which was expressed in Escherichia coli as an N-terminal GST fusion protein. The rP45N'-based ELISA successfully detected P45 antibodies in the sera of 20 ducks immunized with bacterin preparations of R. anatipestifer serotypes 1, 10 15, 19 and the ATCC11845 strain. Antibodies to P45 were also detected in the sera of 25% (75/296) of White Pekin ducks which were imported into Singapore from three different farms. Successful discrimination was obtained between sera from infected ducks and that of specific-pathogen free ducks (p |
| Databáze: | OpenAIRE |
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