Novel Protective Effects of Cistanche Tubulosa Extract Against Low-Luminance Blue Light-Induced Degenerative Retinopathy
| Autor: | Jau-Der Ho, Man Ru Wu, Yu Wen Cheng, George Hsiao, Cheng Hui Lin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Retinal degeneration Cistanche Light Physiology Cistanche tubulosa extract Administration Oral Apoptosis Retinal Pigment Epithelium Protective Agents Retina lcsh:Physiology lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound Western blot In vivo medicine Animals lcsh:QD415-436 Viability assay Phosphorylation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 TUNEL assay lcsh:QP1-981 medicine.diagnostic_test Caspase 3 Plant Extracts Retinal Degeneration JNK Mitogen-Activated Protein Kinases Retinal Hydrogen Peroxide medicine.disease Molecular biology Rats Staining Oxidative Stress Retinal pigment epithelial cells 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 chemistry Terminal deoxynucleotidyl transferase Blue light |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 51, Iss 1, Pp 63-79 (2018) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Blue light-emitting diode light (BLL)-induced phototoxicity plays an important role in ocular diseases and causes retinal degeneration and apoptosis in human retinal pigment epithelial (RPE) cells. Cistanche tubulosa extract (CTE) is a traditional Chinese medicine with many beneficial protective properties; however, few studies have examined the ocular protective roles of CTE. In this study, we investigated the mechanisms underlying the effects of CTE on BLL-induced apoptosis in vitro and in vivo. Methods: RPE cells were applied in the current in vitro study and cell viability was determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis-related protein expression was determined by western blot analysis and immunofluorescence staining. Brown Norway rats were used to examine exposure to commercially available BLL in vivo. Hematoxylin and eosin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and western blot assays were used to examine retinal morphological deformation. Results: CTE significantly inhibited hydrogen peroxide-, tert-butyl hydroperoxide-, sodium azide-, and BLL-induced RPE damage. Further, CTE reduced the expression of apoptotic markers such as cleaved caspase-3 and TUNEL staining after BLL exposure by inactivating apoptotic pathways, as shown via immunofluorescent staining. In addition, CTE inhibited the BLL-induced phosphorylation of c-Jun N-terminal kinase, extra signal-related kinases 1/2, and p38 in RPE cells. In vivo, the oral administration of CTE rescued 60-day periodic BLL exposure-induced decrements in retinal thickness and reduced the number of TUNEL-positive cells in the brown Norway rat model. Conclusion: CTE is a potential prophylactic agent against BLL-induced phototoxicity. |
| Databáze: | OpenAIRE |
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