Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip

Autor: Jean Gamby, Jean-Michel Siaugue, Marie-Charlotte Horny, Vincent Dupuis
Přispěvatelé: Laboratoire Interfaces et Systèmes Electrochimiques (LISE), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre de Nanosciences et de Nanotechnologies (C2N), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), PHysicochimie des Electrolytes et Nanosystèmes InterfaciauX (PHENIX), LabEx MiChem
Rok vydání: 2020
Předmět:
channel microelectrode
Microfluidics
early diagnostics
02 engineering and technology
lcsh:Chemical technology
01 natural sciences
Biochemistry
Analytical Chemistry
law.invention
[SPI]Engineering Sciences [physics]
law
Lab-On-A-Chip Devices
Electrochemistry
lcsh:TP1-1185
core–shell nanoparticles
Instrumentation
ComputingMilieux_MISCELLANEOUS
[PHYS]Physics [physics]
magnetic release
Equipment Design
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
Chip
Atomic and Molecular Physics
and Optics

Inductance
nucleic acids
0210 nano-technology
Nucleic Acid Amplification Techniques
Microfabrication
Superparamagnetism
Materials science
Nanotechnology
010402 general chemistry
Article
[CHIM]Chemical Sciences
Humans
Hyperthermia
Magnetic hyperthermia
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Electrical and Electronic Engineering
Magnetic Phenomena
Lab-on-a-chip
core-shell nanoparticles
Ferrite core
0104 chemical sciences
MicroRNAs
[PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph]
amorphous carbon nitride
Zdroj: Sensors
Sensors, MDPI, 2021, 21 (1), pp.185. ⟨10.3390/s21010185⟩
Sensors (Basel, Switzerland)
Sensors, Vol 21, Iss 185, p 185 (2021)
Volume 21
Issue 1
ISSN: 1424-8220
Popis: The heating of a biologic solution is a crucial part in an amplification process such as the catalytic detection of a biological target. However, in many situations, heating must be limited in microfluidic devices, as high temperatures can cause the denaturation of the chip components. Local heating through magnetic hyperthermia on magnetic nano-objects has opened the doors to numerous improvements, such as for oncology where a reduced heating allows the synergy of chemotherapy and thermotherapy. Here we report on the design and implementation of a lab on chip without global heating of samples. It takes advantage of the extreme efficiency of DNA-modified superparamagnetic core&ndash
shell nanoparticles to capture complementary sequences (microRNA-target), uses magnetic hyperthermia to locally release these targets, and detects them through electrochemical techniques using ultra-sensitive channel DNA-modified ultramicroelectrodes. The combination of magnetic hyperthermia and microfluidics coupled with on-chip electrochemistry opens the way to a drastic reduction in the time devoted to the steps of extraction, amplification and nucleic acids detection. The originality comes from the design and microfabrication of the microfluidic chip suitable to its insertion in the millimetric gap of toric inductance with a ferrite core.
Databáze: OpenAIRE