AICAR Protects Vascular Endothelial Cells from Oxidative Injury Induced by the Long-Term Palmitate Excess
| Autor: | Mikhail V. Samsonov, Nikita V. Podkuychenko, Asker Y. Khapchaev, Eugene E. Efremov, Elena V. Yanushevskaya, Tatiana N. Vlasik, Vadim Z. Lankin, Iurii S. Stafeev, Maxim V. Skulachev, Marina V. Shestakova, Alexander V. Vorotnikov, Vladimir P. Shirinsky |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
malondialdehyde
QH301-705.5 Cell Survival endothelial barrier Palmitates Apoptosis AMP-Activated Protein Kinases Fatty Acids Nonesterified Catalysis Article NO Inorganic Chemistry HUVEC Human Umbilical Vein Endothelial Cells hyperlipidemia Humans Insulin insulin signaling palmitate ROS Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Cells Cultured Organic Chemistry General Medicine Ribonucleotides Aminoimidazole Carboxamide Computer Science Applications Chemistry Oxidative Stress Endothelium Vascular Lipid Peroxidation Insulin Resistance Reactive Oxygen Species Signal Transduction |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 23, Iss 211, p 211 (2022) International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 211 |
| ISSN: | 1422-0067 |
| Popis: | Hyperlipidemia manifested by high blood levels of free fatty acids (FFA) and lipoprotein triglycerides is critical for the progression of type 2 diabetes (T2D) and its cardiovascular complications via vascular endothelial dysfunction. However, attempts to assess high FFA effects in endothelial culture often result in early cell apoptosis that poorly recapitulates a much slower pace of vascular deterioration in vivo and does not provide for the longer-term studies of endothelial lipotoxicity in vitro. Here, we report that palmitate (PA), a typical FFA, does not impair, by itself, endothelial barrier and insulin signaling in human umbilical vein endothelial cells (HUVEC), but increases NO release, reactive oxygen species (ROS) generation, and protein labeling by malondialdehyde (MDA) hallmarking oxidative stress and increased lipid peroxidation. This PA-induced stress eventually resulted in the loss of cell viability coincident with loss of insulin signaling. Supplementation with 5-aminoimidazole-4-carboxamide-riboside (AICAR) increased endothelial AMP-activated protein kinase (AMPK) activity, supported insulin signaling, and prevented the PA-induced increases in NO, ROS, and MDA, thus allowing to maintain HUVEC viability and barrier, and providing the means to study the long-term effects of high FFA levels in endothelial cultures. An upgraded cell-based model reproduces FFA-induced insulin resistance by demonstrating decreased NO production by vascular endothelium. |
| Databáze: | OpenAIRE |
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