In situ detection of mutated hepatitis B virus in microdissected, formalin-fixed liver tissues from patients with chronic hepatitis B
| Autor: | Norio Horiike, Naohiko Kojima, Morikazu Onji, Kojiro Michitaka |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Hepatitis B virus Tissue Fixation Adolescent Hepatitis B virus DNA polymerase medicine.disease_cause Polymerase Chain Reaction Virus Hepatitis B Chronic Orthohepadnavirus Formaldehyde medicine Humans Hepatitis Hepatology biology medicine.diagnostic_test Liver cell Middle Aged biology.organism_classification medicine.disease Virology Hepadnaviridae Liver Liver biopsy DNA Viral Mutation Female |
| Zdroj: | Journal of hepatology. 30(3) |
| ISSN: | 0168-8278 |
| Popis: | Background/Aims: Hepatitis B virus (HBV) quasispecies have been detected in patients with chronic hepatitis B. In order to elucidate the relationship between HBV mutation and liver cell necrosis in situ , we analyzed sublobule-sized specimens microdissected from formalin-fixed paraffin-embedded liver biopsy tissues taken from patients with chronic hepatitis B. Methods: The subjects were 20 patients with chronic hepatitis B. We extracted HBV-DNA from two sublobular regions of HBV-infected liver biopsy tissue, those with the most severe and the mildest hepatitis activity, demonstrated microscopically. The DNA coding sequence of the precore-core region of HBV was determined by amplifying the DNA by the polymerase chain reaction, followed by direct sequencing. Results: In all seven patients with minimal to mild hepatitis activity, but only 4 of 13 with moderate to severe activity, the amino acid sequence of the precore-core region of HBV obtained from the region with the most severe hepatitis activity showed over 99% homology with the corresponding sequence of HBV obtained from region with the mildest hepatitis activity ( p Conclusion: The differences between intrahepatic HBVs observed in patients with highly active hepatitis suggest that exacerbation of hepatitis in vivo is related to the appearance of variants in the precore-core region of HBV. |
| Databáze: | OpenAIRE |
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