An Isocratic Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Benzoyl Peroxide and the Related Compounds Benzoic Acid, Benzaldehyde, Ethyl Benzoate, Methylparaben, and Propylparaben in Dermal Preparations
| Autor: | Muhammad Imran Munir, Aamer Roshanali Khatri, Mohammed Shahid Ali, Syed Rafiuddin, Mohsin Ghori |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Preservative
Spectrophotometry Infrared Parabens Benzoyl peroxide Benzoates Ethyl benzoate Analytical Chemistry Benzaldehyde chemistry.chemical_compound Keratolytic Agents medicine Environmental Chemistry Chromatography High Pressure Liquid Benzoic acid Pharmacology Chromatography Benzoyl Peroxide Methylparaben Benzoic Acid Reference Standards Silane Solutions chemistry Benzaldehydes Indicators and Reagents Chromatography Thin Layer Dermatologic Agents Agronomy and Crop Science Propylparaben Food Science medicine.drug |
| Zdroj: | Journal of AOAC INTERNATIONAL. 91:1025-1036 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/91.5.1025 |
| Popis: | A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of benzoyl peroxide and the related compounds benzoic acid (BA), methylparaben, benzaldehyde, propylparaben, and ethyl benzoate. The compounds are separated on a column containing octadecyl silane chemically bonded to porous silica particles. The mobile phase is acetonitrilebuffer (45 + 55, v/v). Solutions are injected into the chromatographic system under isocratic conditions at a constant flow rate of 1.5 mL/min with UV detection at 235 nm. Analysis of stability samples showed rapid accumulation of BA by thermal degradation. A rationale has been established for the acceptable limit of BA in the formulation, which already contains BA (0.2) as a preservative. The proposed method is efficient and determines the active compound and 5 related compounds in a run time of 20 min. The method was validated according to the guidelines of the International Conference on Harmonization and demonstrated good agreement with the validation requirements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|