Colletotrichum orbiculare MTF4 Is a Key Transcription Factor Downstream of MOR Essential for Plant Signal-Dependent Appressorium Development and Pathogenesis
| Autor: | Takumi Nishiuchi, Yasuyuki Kubo, Sayo Kodama |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Fungus 01 natural sciences Fungal Proteins 03 medical and health sciences Gene Expression Regulation Fungal Colletotrichum Transcription factor Plant Diseases Regulation of gene expression Appressorium biology fungi Colletotrichum orbiculare Fungal genetics food and beverages General Medicine biology.organism_classification Cell biology 030104 developmental biology Mutation Signal transduction Genome Fungal Agronomy and Crop Science 010606 plant biology & botany Signal Transduction Transcription Factors |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 32(3) |
| ISSN: | 0894-0282 |
| Popis: | The cucumber anthracnose fungus Colletotrichum orbiculare forms a specialized infection structure, called an appressorium. Appressorium differentiation relies on fungal perception of physical and biochemical signals at the plant surface. Our previous report showed that the morphogenesis-related NDR (nuclear Dbf2-related) kinase pathway (MOR) is crucial for translating plant-derived signals for appressorium development. Here, we focused on identifying transcriptional regulators downstream of MOR that are involved in plant signal sensing and transduction for appressorium development. Based on whole-genome transcript profiling, we identified a Zn(II)2Cys6 transcription factor, CoMTF4, as a potential downstream factor of MOR. CoMTF4 was expressed in planta rather than in vitro under the control of the NDR kinase CoCbk1. Phenotypes of comtf4 mutants, strains with constitutively active CoCbk1 and strains with constitutive overexpression of CoMTF4 suggested that CoMtf4 acts downstream of MOR. Furthermore, nuclear localization of CoMtf4 was dependent on the MOR and responsive to plant-derived signals that lead to appressorium morphogenesis. Thus, we conclude that CoMtf4 is a transcription factor downstream of MOR that is essential for appressorium morphogenesis and pathogenesis and is regulated in response to plant-derived signals. This study provides insights into fungal sensing of plant signals and subsequent responses critical for appressorium formation. |
| Databáze: | OpenAIRE |
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