Terrestrosin D from Tribulus terrestris attenuates bleomycin-induced inflammation and suppresses fibrotic changes in the lungs of mice
| Autor: | Quanli Liu, Ming An, Mengni Bian, Min Qiu, Changxiao Liu, Shunbang Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Pharmaceutical Science Context (language use) RM1-950 Bleomycin 030226 pharmacology & pharmacy 01 natural sciences anti-fibrosis pharmaceutical 03 medical and health sciences Hydroxyproline chemistry.chemical_compound 0302 clinical medicine Fibrosis Internal medicine Drug Discovery Pulmonary fibrosis medicine hydroxyproline Pharmacology Lung medicine.diagnostic_test pulmonary fibrosis business.industry bleomycin-induced pulmonary inflammation General Medicine medicine.disease anti-inflammatory agent 0104 chemical sciences 010404 medicinal & biomolecular chemistry Bronchoalveolar lavage medicine.anatomical_structure Endocrinology Complementary and alternative medicine chemistry Molecular Medicine Histopathology Therapeutics. Pharmacology business |
| Zdroj: | Pharmaceutical Biology, Vol 57, Iss 1, Pp 694-700 (2019) |
| ISSN: | 1744-5116 1388-0209 |
| Popis: | Context: Terrestrosin D (TED), from Tribulus terrestris L. (Zygophyllaceae), exhibits anti-tumour and anti-inflammatory activities. However, its effects on bleomycin (BLM)-induced pulmonary inflammation and the subsequent fibrotic changes remain unclear. Objective: To examine the anti-inflammatory and anti-fibrotic effects of TED against BLM in murine pulmonary tissues. Materials and methods: Male SPF mice received saline (control), TED (10 mg/kg), BLM (2.5 mg/kg), or BLM (2.5 mg/kg) + TED (10 mg/kg) group. BLM was administered as a single intranasal inoculation, and TED was intraperitoneally administered once daily. After 2 and 6 weeks of treatment, cell number and differentiation (Giemsa staining) and TNF-α, IL-6, IL-8, TGF-β1, and PDGF-AB levels (ELISA) were determined in the bronchoalveolar lavage fluid (BALF). Hydroxyproline (Hyp) content in the left pulmonary tissue was also determined (ELISA). The right pulmonary tissue was H&E-stained and assessed for the severity of pulmonary fibrosis using the Ashcroft scoring method. Compared with the BLM group, TED decreased inflammatory cell infiltration; number of macrophages (p |
| Databáze: | OpenAIRE |
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