Inhibition of Urease, Elastase, and β-Glucuronidase Enzymatic Activity by Applying Aqueous Extracts of Opuntia oligacantha C.F. Först Acid Fruits: In Vitro Essay under Simulated Digestive Conditions
| Autor: | Armando Peláez-Acero, Laura Peralta-Adauto, Gabriela Medina-Pérez, Laura N. Afanador-Barajas, Fabián Fernández-Luqueño, Elizabeth Pérez-Soto, Rafael G. Campos-Montiel |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
Urease QH301-705.5 QC1-999 Alcohol phenols chemistry.chemical_compound tannins General Materials Science Phenols Food science Biology (General) QD1-999 Instrumentation Fluid Flow and Transfer Processes chemistry.chemical_classification Opuntia sp bioactive compounds Aqueous solution biology Chemistry Physics Process Chemistry and Technology Elastase General Engineering in vitro Engineering (General). Civil engineering (General) In vitro Computer Science Applications Glucuronidase enzymatic inhibition Enzyme betalains flavonoids biology.protein TA1-2040 |
| Zdroj: | Applied Sciences, Vol 11, Iss 7705, p 7705 (2021) Applied Sciences Volume 11 Issue 16 |
| ISSN: | 2076-3417 |
| Popis: | Non-communicable diseases such as gastric inflammatory diseases and the hepatic pathologies are mainly related to bad lifestyle habits such as recurrent consumption of non-steroidal anti-inflammatory drugs (NSAIDs), excessive intake of alcohol, tobacco, steroids (high doses), alkaline agents, strong acid foods, and high-fat food, and Helicobacter pylori infections, among others. The fruit of Opuntia oligacantha C.F. Först var. Ulapa (xoconostle) is currently being studied due its nutritional and functional properties. The objective of the present study was to evaluate gastroprotective, anti-inflammatory, and hepatoprotective activities of different parts of xoconostle fruit by establishing in vitro simulated gastrointestinal conditions. Four treatments were established to test aqueous extracts (pericarp (P), mesocarp (M), endocarp (E) and whole fruit (W)). The quantified bioactive compounds were the total phenols, flavonoids, tannins, and betalains. The enzymatic assays were: urease, elastase, and β-glucuronidase. Significant differences (p < 0.05) of bioactive compounds content were measured in xoconostle extracts, the highest concentration was found in W (phenols 313 mg GAE/100 g, flavonoids 189 mg QE/100 g, tannins 71 mg CATE/100 g). The betalains content was higher in E 17 mg/100 g significant differences were observed (p < 0.05) in the enzymatic inhibitions test (urease, elastase and β-glucuronidase), where W presented the highest inhibition activity (86%, 79%, and 84%), respectively. Bioactive compounds after in vitro gastrointestinal tests were maintained above 60% enzymatic inhibition activity. |
| Databáze: | OpenAIRE |
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