A study of the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system of umbilical vein endothelial cells
| Autor: | Yanyi Liu, Yuan Su, Xiaohui Fan, Xueyang Zhang, Fei Hu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Umbilical Veins
Biomedical Engineering Biophysics Health Informatics Bioengineering umbilical vein endothelial cells 02 engineering and technology Bioceramic Cell morphology Umbilical vein Biomaterials Andrology 03 medical and health sciences Western blot Human Umbilical Vein Endothelial Cells medicine Humans Cytotoxicity 030304 developmental biology 0303 health sciences medicine.diagnostic_test Plant Extracts Cell growth Chemistry 021001 nanoscience & nanotechnology Cell counting Receptor TIE-2 Durapatite Tie2 Real-time polymerase chain reaction Ang embryonic structures cardiovascular system hydroxyapatite bioceramics extract 0210 nano-technology Research Article Information Systems |
| Zdroj: | Technology and Health Care |
| ISSN: | 1878-7401 0928-7329 |
| Popis: | OBJECTIVE: We aimed to investigate the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system and cell proliferation of umbilical vein endothelial cells. METHODS: Human umbilical vein endothelial cells (HUVECs) were used in this research. There are two induvial groups, control group and hydroxyapatite bioceramics extract treatment group. Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation. Western blot and real time quantitative PCR (Q-PCR) were used to evaluate the protein and mRNA expression levels of Ang1, Ang2 and Tie2 in Ang/Tie2 system, respectively. All the results were statistically analyzed by Spss19.0. All data were presented as mean ± standard error of mean (SEM). Student’s t-test was performed to determine the differences among grouped data. RESULTS: Hydroxyapatite bioceramics extract showed no effect on the cell morphology and cell proliferation of HUVECs. Interestingly, we found that both Ang2 and Tie2 protein and mRNA level were markedly increased by hydroxyapatite bioceramics extract. CONCLUSIONS: Hydroxyapatite bioceramic extract showed no cytotoxicity to HUVECs, and might regulate vascular remodeling by mediating Ang/Tie2 system. |
| Databáze: | OpenAIRE |
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