Analysis on sarcoglycans expression as markers of septic cardiomyopathy in sepsis-related death
| Autor: | Giovanna Vermiglio, Alessio Asmundo, Cristina Mondello, Giuseppina Rizzo, Debora Di Mauro, Elvira Ventura Spagnolo, Angela Alibrandi, Elena Filippini |
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| Přispěvatelé: | Ventura Spagnolo, E, Mondello, C, Di Mauro, D, Vermiglio, G, Asmundo, A, Filippini, E, Alibrandi, A, Rizzo, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Forensic pathology Sepsi Immunofluorescence Forensic pathology Immunofluorescence Sarcoglycans Sepsis Septic cardiomyopathy Autopsy 030204 cardiovascular system & hematology Pathology and Forensic Medicine Sepsis 03 medical and health sciences 0302 clinical medicine Settore MED/43 - Medicina Legale Retrospective Studie Sarcoglycans medicine Humans Sarcoglycan Fluorescent Antibody Technique Indirect Retrospective Studies Cause of death Aged Cardiomyopathie business.industry Myocardium Organ dysfunction Case-control study Biomarker medicine.disease Pathophysiology 030104 developmental biology Septic cardiomyopathy Case-Control Studies Female medicine.symptom Cardiomyopathies business Case-Control Studie Biomarkers Human |
| Popis: | The post-mortem assessment of sepsis-related death can be carry out by many methods recently suggested as microbiological and biochemical investigations. In these cases, the cause of death is a multiple organ dysfunction due to a dysregulated inflammatory response occurring after the failure of infection control process. It was highlighted also that the heart can be a target organ in sepsis which determines the so-called septic cardiomyopathy characterized by myocardial depression. Several mechanisms to explain the pathophysiology of septic cardiomyopathy were suggested, but very few studies about the structural alterations of cardiac cells responsible for myocardial depression were carried out. The aim of this study was to evaluate whether sarcoglycans (SG) were involved in septic cardiac damage analyzing their expression in sepsis-related deaths and, particularly, if these proteins can be used as markers of septic myocardial dysfunction. Cases of septic-related death confirmed by clinical and autopsy records were investigated and compared to a control group of traumatic deaths. Indirect immunofluorescence analysis was performed to analyze α-SG, β-SG, δ-SG, ζ-SG, ε-SG, and γ-SG. Decrease of fluorescence staining pattern for all tested sarcoglycans was observed in the septic-related deaths compared to normal fluorescence staining pattern of control group. These results provide new findings about the myocytes structural alterations due to sepsis and suggest that these proteins could be used in forensic assessment of septic cardiomyopathy. |
| Databáze: | OpenAIRE |
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