The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish
| Autor: | Karl Wah Keung Tsim, Tina T. X. Dong, Qiwei Qin, Jian Xiao, Shao-Ping Li, Wen-Xiong Wang, Qiyun Wu, Yi-Teng Xia, Ran Duan, Wei-Hui Hu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Programmed cell death Anti-Inflammatory Agents Inflammation Aquatic Science Article Siganus fuscescens Cell Line Proinflammatory cytokine Fish Diseases 03 medical and health sciences Traditional Chinese medicine Downregulation and upregulation Anti-inflammation medicine Animals Environmental Chemistry Hypoxia biology Plant Extracts Rabbit fish NF-kappa B 04 agricultural and veterinary sciences General Medicine Hypoxia-Inducible Factor 1 alpha Subunit biology.organism_classification Molecular biology Perciformes 030104 developmental biology Cell culture 040102 fisheries 0401 agriculture forestry and fisheries Scutellaria baicalensis medicine.symptom Immunostaining Anti-hypoxia Signal Transduction |
| Zdroj: | Fish & Shellfish Immunology |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.07.050 |
| Popis: | A new cell line derived from dorsal fin of rabbit fish Siganus fuscescens was developed and characterized. The cell line was isolated from the dorsal fin, named as rabbit fish fin (RFF) cell line, and which was sub-cultured for 50 cycles since the development. This cell line was tested for growth in different temperatures and serum concentrations, and the best growing condition was at 20% serum at 28 °C. In cultured RFF cells, amplification of 18S rRNA from genomic DNA and immunostaining of cellular cytokeratin confirmed the proper identity of S. fuscescens fish. After 30th passage of cultures, the cells were exposed to challenge of inflammation, triggered by LPS, and hypoxia, mimicked by CoCl2. Cultured RFF cells showed robust sensitive responses to inflammation and hypoxia in directing the expressions of cytokines and hypoxia inducible factor-1α (HIF-1α). The water extract of aerial part of Scutellaria baicalensis (SBA) has been shown in rabbit fish to prevent inflammation. Here, we extended this notion of testing the efficacy of SBA extract in the developed cultured RFF cells. Application of SBA extract inhibited the expression of LPS-induced inflammatory cytokines, i.e. IL-1β, IL-6, as well as the signaling of NF-κB. The application of CoCl2 in cultured RFF cells triggered the hypoxia-induced cell death and up regulation of HIF-1α. As expected, applied SBA extract in the cultures prevented the hypoxia-induced signaling. Our results show the established RFF cell line may be served as an ideal in vitro model in drug screening relating to inflammation and hypoxia. Additionally, we are supporting the usage of SBA herbal extract in fish aquaculture, which possesses efficacy against inflammation and hypoxia. Highlights • A new cell line divided from Siganus fuscescens was established and named as RFF cell. • The RFF cell exhibited sensitive responses to LPS-induced inflammation and hypoxia environment. • The aerial part of S. baicalensis extract showed anti-inflammatory and anti-hypoxia effects on RFF cell. |
| Databáze: | OpenAIRE |
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