Hexylacrylate-based mixed-mode monolith, a stationary phase for the nano-HPLC separation of structurally related enkephalins
| Autor: | Claire Smadja, Jordi Rodrigo, Gaëlle Proczek, Ayat Abbood, Myriam Taverna, Stéphanie Descroix, Christine Herrenknecht |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Neurotransmitter Agents geography Chromatography geography.geographical_feature_category Molecular Structure Peptide Enkephalins Sulfonic acid Mixed mode Biochemistry High-performance liquid chromatography Analytical Chemistry Resins Synthetic chemistry Stationary phase Phase (matter) Nano Nanotechnology Adsorption Monolith Chromatography High Pressure Liquid |
| Zdroj: | Analytical and Bioanalytical Chemistry. 400:459-468 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-011-4762-4 |
| Popis: | The potential of an in situ photopolymerized hexylacrylate-based monolithic stationary phase-bearing sulfonic acid groups was investigated by studying the chromatographic retention of small structurally related peptides (enkephalins) by nano-LC. Several retention mechanisms were highlighted. First, a reverse-phase chromatographic behavior toward neutral solutes due to hexylacrylate-moieties was demonstrated. Second, an evaluation of the influences of buffer pH suggested the involvement of a cation-exchange mechanism due to the presence of 2-acrylamido-2-methyl-1-propanesulfonic acid. This cation-exchange phenomenon was confirmed by the clear influence of Na(+) concentration in the mobile phase on peptide retention. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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