Differential scanning calorimetry, biochemical, and biomechanical analysis of human skin from individuals with diabetes mellitus
| Autor: | Mahmoud Melling, Christa Zangerle, Grazyna Sobal, Wolfgang Pfeiler, Martina Schnallinger, Daniela Karimian-Teherani, Ernst J. Menzel |
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| Rok vydání: | 2000 |
| Předmět: |
Glycation End Products
Advanced Dorsum Protein Denaturation medicine.medical_specialty Human skin Collagen denaturation Differential scanning calorimetry Glycation Internal medicine Diabetes mellitus medicine Humans Aged Skin Aged 80 and over Calorimetry Differential Scanning integumentary system business.industry Lysine medicine.disease Agricultural and Biological Sciences (miscellaneous) Biomechanical Phenomena Surgery Endocrinology Unit mass Diabetes Mellitus Type 2 Anisotropy Female Collagen Anatomy business Heat flow |
| Zdroj: | The Anatomical Record. 259:327-333 |
| ISSN: | 1097-0185 0003-276X |
| Popis: | The aim of this work was to compare biochemical, two-dimensional biomechanical and calorimetric parameters of diabetic skin vs. control skin. Skin specimens taken from the palms and backs of the hands of aged persons with non-insulin-dependent diabetes mellitus (NIDDM) and of controls (CO) were compared (age range 68–85 years). Only skin specimens from individuals with diabetes mellitus (DM) showed an increased fluorescence specific for the formation of advanced glycation end-products (AGEs) and the presence of tissue AGEs, such as Ne-(Carboxymethyl)lysine (CML). Differential scanning calorimetry (DSC) revealed an elevation of the heat flow per unit mass during collagen denaturation in diabetic skin samples. However, the temperatures of the heat flow maximum and the onset of the phase transformation were not uniformly altered. Young′s moduli were found to be increased in diabetic skin and correlated with AGE-fluorescence and tissue AGEs. The ratio between the Young's moduli, which defines a measure for the degree of anisotropy, was higher for dorsal skins from hands. In dorsal skin specimens from diabetic subjects the degree of anisotropy was more pronounced than in healthy controls. In general, neither of the measured parameters showed any correlation with age. However, E1 moduli were clearly associated with the duration of diabetes. Anat Rec 259:327–333, 2000. © 2000 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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