TGF-β1 induces the formation of vascular-like structures in embryoid bodies derived from human embryonic stem cells
| Autor: | Wen‑yu Zhong, Yan Wang, De Jian Qian, Jun‑hong Lu, Ju Liu, Yi Lin Cao, Xiang‑kai Guo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
CD31
Cancer Research embryoid body Cell transforming growth factor-β General Medicine Embryoid body Articles human embryonic stem cell Biology Bioinformatics Embryonic stem cell Cell biology Endothelial stem cell medicine.anatomical_structure Immunology and Microbiology (miscellaneous) Extracellular medicine endothelial cell Inner cell mass Transforming growth factor |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Human embryonic stem cells (ESCs) can differentiate into endothelial cells in response to stimuli from extracellular cytokines. Transforming growth factor (TGF)-β1 signaling is involved in stem cell renewal and vascular development. Previously, human ESCs were isolated from inner cell mass and a stable ESC line was developed. In the present study, the effects of extracellular TGF-β1 were investigated on human ESC-derived embryoid bodies (EB) in suspension. The structures of the EBs were analyzed with light and electron microscopy, while the cellular composition of the EBs was examined via the expression levels of specific markers. Vascular-like tubular structures and cardiomyocyte-like beating cells were observed in the EBs at day 3 and 8, respectively. The frequencies of vascular-like structures and beating cells in the TGF-β1 treated group were significantly higher compared with the control group (84.31 vs. 12.77%; P |
| Databáze: | OpenAIRE |
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