Protistan parasite QPX of hard-shell clam Mercenaria mercenaria is a member of Labyrinthulomycota
| Autor: | Sharon E. McGladdery, Greg S. MacCallum, Robin R. Gutell, Jamie J. Cannone, Colleen A. Murphy, Mark A. Ragan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Molecular Sequence Data
Aquatic Science Polymerase Chain Reaction 18S ribosomal RNA Microbiology Phylogenetics RNA Ribosomal 18S Animals Parasite hosting New Brunswick Ribosomal DNA Phylogeny Ecology Evolution Behavior and Systematics Electrophoresis Agar Gel Mercenaria Base Sequence biology Ecology Eukaryota Sequence Analysis DNA DNA Protozoan Ribosomal RNA Bivalvia biology.organism_classification Protozoa Sequence Alignment |
| Zdroj: | Diseases of Aquatic Organisms. 42:185-190 |
| ISSN: | 1616-1580 0177-5103 |
| DOI: | 10.3354/dao042185 |
| Popis: | Biomass of the protistan parasite QPX (quahaug parasite X) of hard-shell clam Mercenaria mercenaria was enriched from in vitro culture. The nuclear gene encoding the 18S RNA of the small-subunit ribosomal (ssu-rDNA) was recovered using the polymerase chain reaction (PCR) and sequenced. Phylogenetic analysis clearly showed that QPX is a member of phylum Labyrinthulomycota, within which it appears as a specific relative of Thraustochytrium pachydermum. These results confirm the provisional assignment of QPX to the Labyrinthulomycota made previously on the basis of morphological and ultrastructural characters found in some, but not all, geographic isolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|