Protective effect of pterostilbene in a streptozotocin‐induced mouse model of Alzheimer's disease by targeting monoamine oxidase B
| Autor: | Qiushi Li, Xidong Li, Buxian Tian, Long Chen |
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| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Amyloid beta-Peptides Caspase 3 Interleukin-6 Superoxide Dismutase Tumor Necrosis Factor-alpha NF-kappa B Neurodegenerative Diseases Toxicology Streptozocin Mice Inbred C57BL Disease Models Animal Mice Alzheimer Disease Resveratrol Stilbenes Animals Humans Reactive Oxygen Species Monoamine Oxidase Aged bcl-2-Associated X Protein |
| Zdroj: | Journal of Applied Toxicology. 42:1777-1786 |
| ISSN: | 1099-1263 0260-437X |
| Popis: | Alzheimer's disease (AD) is a neurodegenerative disease in elderly population. Pterostilbene (PTS) is a resveratrol analog with neuroprotective activity. However, the biological mechanisms of PTS in AD progression are largely uncertain. An animal model of AD was established using streptozotocin (STZ)-treated C57BL/6J mice. Monoamine oxidase B (MAOB) expression was analyzed by bioinformatics analysis and detected by western blotting assay. The memory impairment was investigated by Morris water maze test. The levels of Tau hyperphosphorylation and death-related proteins were detected by western blotting analysis. The levels of amyloid β (Aβ)sub1-42/subaccumulation, oxidative stress-related markers (ROS, MDA, SOD, and GSH), and inflammation-relative markers (TNF-α, IL-1β, IL-6, and p-NF-κB) were measured by ELISA. MAOB expression was increased in hippocampus of AD mice, and it was decreased by PTS. PTS attenuated STZ-induced body weight loss and memory impairment by regulating MAOB. PTS mitigated Aβsub1-42/subaccumulation and Tau hyperphosphorylation by regulating MAOB in STZ-treated mice. PTS attenuated neuronal death by decreasing cleaved caspase-3 and Bax levels and increasing Bcl2 expression in hippocampus by regulating MAOB in STZ-treated mice. PTS weakened STZ-induced oxidative stress in hippocampus by decreasing ROS and MDA levels and increasing SOD and GSH levels by regulating MAOB. PTS protected against STZ-induced neuroinflammation in hippocampus by inhibiting TNF-α, IL-1β, IL-6, and p-NF-κB levels through regulating MAOB. In conclusion, PTS alleviates STZ-induced memory impairment, Aβsub1-42/subaccumulation, Tau hyperphosphorylation, neuronal death, oxidative stress, and inflammation by decreasing MAOB in AD mice, proving anti-AD potential of PTS. |
| Databáze: | OpenAIRE |
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