Using Scanning Electron Microscopy and Atomic Force Microscopy to Study the Formation of Nanoparticles on Red Blood Cell Surface in Cervical Cancer Patients
| Autor: | Nyurguyna Fedorova, Nadezhda Nikolaevа, A. N. Pavlov, Michael Ruzhansky, Georgy V. Maksimov, I.V. Kononova, Daiaana Barashkova, Junqing Huang, S.N. Mamaeva, Petr V. Nikiforov, Motrena Semenova, Lyubov Frolova |
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| Rok vydání: | 2020 |
| Předmět: |
Morphology (linguistics)
Scanning electron microscope medicine.medical_treatment Brachytherapy lcsh:Medicine red blood cell radiation therapy General Biochemistry Genetics and Molecular Biology Nuclear magnetic resonance morphology 0502 economics and business medicine General Immunology and Microbiology Chemistry General Neuroscience Vesicle lcsh:R 05 social sciences nanoscale Venous blood Extracellular vesicle Radiation therapy Red blood cell medicine.anatomical_structure 050211 marketing extracellular vesicle 050203 business & management |
| Zdroj: | International Journal of Biomedicine, Vol 10, Iss 1, Pp 70-75 (2020) |
| ISSN: | 2158-0529 2158-0510 |
| DOI: | 10.21103/article10(1)_oa12 |
| Popis: | Background: In this study, we used scanning electron microscopy (SEM) and atomic force microscopy (AFM) to examine the changes in morphology of red blood cells (RBCs) and to investigate the nanoparticles (NPs) found on their surface in cervical cancer (CC) patients undergoing radiation therapy (RT). Methods and Results: We obtained smears of venous blood from 12 CC patients at the start, midway and at the end of external beam RT and then midway and at the end of brachytherapy. It was found that in CC patients, the number of RBCs with abnormal morphology increased and NPs appeared on their surface. During RT, the total number of abnormally shaped RBCs and the number and size of NPs increased. The RBC diameter was 8.38 +/- 0.36 mu m in the control group and 9.41 +/- 0.47 mu m in CC patients. The average diameter of NPs on the RBC surface was 69.91 +/- 12.15 nm and their average height 23.75 +/- 3.70 nm. After RT, the morphology of RBCs was restored, and the formation of NPs decreased. Conclusion: The changes observed could serve as the basis for developing efficacy indicators of cancer radiation therapy. |
| Databáze: | OpenAIRE |
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