Cloning and sequencing of the glucosyl transferase-encoding gene from converting bacteriophage X (SFX) of Shigella flexneri
| Autor: | Donna J. Verma, Pham Thi Huan, Naresh K. Verma, Alf A. Lindberg |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Viral Molecular Sequence Data Biology Molecular cloning Microbiology Shigella flexneri Bacteriophage Genetics Bacteriophages Amino Acid Sequence Cloning Molecular Gene Cloning Viral Structural Proteins Base Sequence Polysaccharides Bacterial Nucleic acid sequence General Medicine biology.organism_classification Enterobacteriaceae Molecular biology Carbohydrate Sequence Glucosyltransferases biology.protein Glucosyltransferase |
| Zdroj: | Gene. 129(1) |
| ISSN: | 0378-1119 |
| Popis: | Summary Shigella flexneri type Y strains (−;3,4) are converted to type X (−;7,8) by bacteriophage X (SFX) that causes glucosylation of the O-antigenic polysaccharide chain. The gene (gtr) encoding glucosyl transferase from bacteriophage X has been cloned and sequenced. The protein encoded by gtr consists of 416 amino acids with a Mr of 47369. The cloned gtr product was able to convert a S. flexneri strain type Y (SFL 124, a live attenuated candidate vaccine strain) to type X. The importance of the hybrid strain in vaccine development is discussed. |
| Databáze: | OpenAIRE |
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