Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression
| Autor: | Linwen Zeng, Shaofeng Yuan, Pengfei Zhou, Jianming Gong, Xiangdong Kong, Ming Wu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A VEGFA endocrine system endocrine system diseases Down-Regulation Bioengineering Applied Microbiology and Biotechnology miR-195 Cell Movement Cell Line Tumor circPVT1 Humans Neoplasm Invasiveness Thyroid Neoplasms Wnt Signaling Pathway Cell Proliferation Base Sequence RNA Circular General Medicine Middle Aged malignant progression Gene Expression Regulation Neoplastic MicroRNAs Thyroid Cancer Papillary Papillary thyroid carcinoma Female TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered article-version (VoR) Version of Record Bioengineered, Vol 12, Iss 2, Pp 11795-11810 (2021) |
| ISSN: | 2165-5987 2165-5979 |
| DOI: | 10.1080/21655979.2021.2008639 |
| Popis: | Circular RNAs (circRNAs) have been reported to be involved in the progression of papillary thyroid carcinoma (PTC). However, the role of circular RNA Pvt1 oncogene (circPVT1) in PTC has rarely been reported. In this study, we aimed to investigate the function and mechanism of circPVT1 in PTC. The expression level of circPVT1, miR-195 and VEGFA were determined by reverse transcription‑quantitative PCR (RT‑qPCR). Fisher’s exact test was used to analyze the correlation between circPVT1 expression and PTC clinical features. Cell Counting Kit-8 (CCK-8) and 5-Ethynyl-2ʹ-deoxyuridine (EdU) staining assay and transwell assay were conducted to evaluate the cell proliferation, migration and invasion ability. Dual-luciferase reporter and Western blot assay were conducted for evaluating the correlation between miR-195 and circPVT1 or VEGFA. The results of RT-PCR showed that the expression level of circPVT1 was significantly upregulated in PTC tissues and cell lines. After downregulating circPVT1 expression in PTC cells, the abilities of cell proliferation, migration, and invasion were obviously suppressed, and the Wnt/β-catenin signaling pathway was also repressed. Besides, miR-195 could both bind to PVT1 and VEGFA, while PVT1 could promote the expression of VEGFA by binding to miR-195. Downregulation of VEGFA expression in PTC cells revealed weakened cell proliferation, migration, and invasion capacities, and restrained Wnt/β-catenin signaling pathway. Therefore, we demonstrated that circPVT1 could promote VEGFA expression by sponging miR-195. CircPVT1 could serve as a molecule sponge for miR-195 and mediate the ceRNA network to promote the expression of VEGFA, thus contributed to the malignant progression of PTC. |
| Databáze: | OpenAIRE |
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