DIFFERENTIAL EFFECTS OF TRYPTOPHAN PHOTOPRODUCTS AND H2O2ON THE GROWTH OF MOUSE EMBRYONIC FIBROBLASTS
| Autor: | Seymour Zigman, D. Ennist, T. Yulo, J. D. Hare |
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| Rok vydání: | 1978 |
| Předmět: |
DNA synthesis
Ultraviolet Rays Tryptophan chemistry.chemical_element Hydrogen Peroxide General Medicine Fibroblasts Biology Embryo Mammalian Biochemistry Differential effects Embryonic stem cell Oxygen Mice chemistry.chemical_compound chemistry Yield (chemistry) Animals Physical and Theoretical Chemistry Hydrogen peroxide DNA |
| Zdroj: | Photochemistry and Photobiology. 27:281-284 |
| ISSN: | 1751-1097 0031-8655 |
| Popis: | Exposure of dilute solutions of L-tryptophan in balanced salt mixtures to near-ultraviolet light (ca. 320–390nm) yield several photoproducts capable of inhibiting the growth of cultured mouse embryonic fibroblasts. Recent literature suggested a need to test the possibility that the major toxic photoproduct is hydrogen peroxide, and therefore several lines of experimentation were pursued. On the basis of chemical and morphological studies and an analysis of the uptake of 3H-thymidine by these cells and its incorporation into their DNA, it is concluded that sufficient hydrogen peroxide could not be generated without the addition of high concentrations of oxygen to influence the growth or DNA synthesis of mouse embryonic fibroblasts. |
| Databáze: | OpenAIRE |
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