Up-regulated interleukin-4 production by peripheral T-helper cells in idiopathic membranous nephropathy
| Autor: | Yuji Kudoh, Masatomo Taniguchi, Hideki Hirakata, Kyoichi Fukuda, Hideki Yotsueda, Kohsuke Masutani, Masanori Tokumoto, Hidetoshi Kanai, Mitsuo Iida, Hitoshi Nakashima, Kazuhiko Tsuruya |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male medicine.medical_specialty Helper T lymphocyte medicine.medical_treatment Glomerulonephritis Membranous Interferon-gamma Focal segmental glomerulosclerosis Membranous nephropathy Internal medicine medicine Humans Lymphocyte Count Interleukin 4 Aged Transplantation Glomerulosclerosis Focal Segmental business.industry Interleukin T-Lymphocytes Helper-Inducer Middle Aged medicine.disease Up-Regulation Proteinuria Endocrinology Cytokine Nephrology Case-Control Studies Immunoglobulin G Immunology Nephrosis Female Interleukin-4 business Immune complex disease |
| Zdroj: | Nephrology Dialysis Transplantation. 19:580-586 |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | BACKGROUND Helper T (Th) cells are classified into Th1 and Th2 subsets based on cytokine production and the Th1/Th2 paradigm explains differences in inflammatory effector pathways in various human diseases. Membranous nephropathy (MN) is an immune complex disease associated with Th2 nephritogenic immune response. However, overproduction of interleukin (IL)-4, a principal Th2 cytokine, has not been demonstrated. We investigated Th1/Th2 cytokine production by peripheral Th cells and its association with the degree of proteinuria in MN. METHODS We analysed production of Th1/Th2 cytokines, interferon (IFN)-gamma and IL-4 by peripheral Th cells, using an intracellular cytokine detection method with flow cytometry in patients with MN (n = 24). The data were compared with data from healthy subjects (n = 51), subjects with minimal change nephrotic syndrome (MCNS; n = 13) and subjects with focal segmental glomerulosclerosis (FSGS; n = 12). We compared the percentages of IFN-gamma+ and IL-4+ Th cells and the peripheral Th1/Th2 ratio (IFN-gamma/IL-4 ratio) among the four groups. We also examined the association of IFN-gamma and IL-4 production with clinical parameters of MN. RESULTS The mean percentage of IL-4+ cells in MN (3.9+/-1.2%) was significantly higher than in the control (2.4+/-1.0%), MCNS (2.3+/-1.4%) and FSGS (2.3+/-1.2%) groups (P |
| Databáze: | OpenAIRE |
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