Details of the Collagen and Elastin Architecture in the Human Limbal Conjunctiva, Tenon's Capsule and Sclera Revealed by Two-Photon Excited Fluorescence Microscopy
| Autor: | Choul Yong Park, Ji-won Kwon, Jason A. Liao, Jimmy K. Lee, Roy S. Chuck, Catherine M. Marando |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Materials science Conjunctiva Tenon Capsule 01 natural sciences 010309 optics 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional Two-photon excitation microscopy Tenon's capsule 0103 physical sciences Microscopy medicine Fluorescence microscope Cadaver Humans Aged biology Reproducibility of Results Anatomy Middle Aged eye diseases Sclera Elastin Autofluorescence medicine.anatomical_structure Microscopy Fluorescence Multiphoton 030221 ophthalmology & optometry biology.protein Female sense organs Collagen Biomedical engineering |
| Zdroj: | Investigative ophthalmologyvisual science. 57(13) |
| ISSN: | 1552-5783 |
| Popis: | Purpose To investigate the architecture and distribution of collagen and elastin in human limbal conjunctiva, Tenon's capsule, and sclera. Methods The limbal conjunctiva, Tenon's capsule, and sclera of human donor corneal buttons were imaged with an inverted two-photon excited fluorescence microscope. No fixation process was necessary. The laser (Ti:sapphire) was tuned at 850 nm for two-photon excitation. Backscatter signals of second harmonic generation (SHG) and autofluorescence (AF) were collected through a 425/30-nm and a 525/45-nm emission filter, respectively. Multiple, consecutive, and overlapping (z-stack) images were acquired. Collagen signals were collected with SHG, whereas elastin signals were collected with AF. Results The size and density of collagen bundles varied widely depending on depth: increasing from conjunctiva to sclera. In superficial image planes, collagen bundles were |
| Databáze: | OpenAIRE |
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