Motion-free endoscopic system for brain imaging at variable focal depth using liquid crystal lenses
| Autor: | Armen Saghatelyan, Tigran Galstian, Arutyun Bagramyan |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
General Physics and Astronomy Neuroimaging 02 engineering and technology 01 natural sciences General Biochemistry Genetics and Molecular Biology 010309 optics Optics Liquid crystal 0103 physical sciences Animals Constant voltage General Materials Science Lenses business.industry General Engineering Brain Endoscopy Equipment Design General Chemistry 021001 nanoscience & nanotechnology Liquid Crystals Mice Inbred C57BL 0210 nano-technology business Focal shift |
| Zdroj: | Journal of Biophotonics. 10:762-774 |
| ISSN: | 1864-063X |
| DOI: | 10.1002/jbio.201500261 |
| Popis: | We present a motion-free system for microendoscopic imaging of biological tissues at variable focal depths. Fixed gradient index and electrically tunable liquid crystal lenses (TLCL) were used to build the imaging optical probe. The design of the TLCL enables polarization-independent and relatively low-voltage operation, significantly improving the energy efficiency of the system. A focal shift of approximately 74 ± 3 µm could be achieved by electrically controlling the TLCL using the driving frequency at a constant voltage. The potential of the system was tested by imaging neurons and spines in thick adult mouse brain sections and in vivo, in the adult mouse brain at different focal planes. Our results indicate that the developed system may enable depth-variable imaging of morpho-functional properties of neural circuitries in freely moving animals and can be used to investigate the functioning of these circuitries under normal and pathological conditions. |
| Databáze: | OpenAIRE |
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