New glucose oxidase-immobilized stimuli-responsive dextran nanoparticles for insulin delivery
| Autor: | Rohini Dharela, Bhagat Ram, Sunita Ranote, Shivani Jamwal, Ghanshyam S. Chauhan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
02 engineering and technology engineering.material Biochemistry Glucose Oxidase 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems X-Ray Diffraction Structural Biology Spectroscopy Fourier Transform Infrared medicine Humans Insulin Glucose oxidase Carbon-13 Magnetic Resonance Spectroscopy Particle Size Pharmaceutical sciences Molecular Biology 030304 developmental biology 0303 health sciences biology Chemistry Dextrans General Medicine Hydrogen-Ion Concentration Enzymes Immobilized 021001 nanoscience & nanotechnology Controlled release Drug Liberation Kinetics Glucose Dextran Drug delivery engineering biology.protein Biophysics Nanoparticles Biopolymer Nanocarriers 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 123:968-978 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.11.147 |
| Popis: | Designing strategies for the use of biopolymer-based nanoparticles as drug delivery carriers is a considerable challenge in pharmaceutical science. Present study reports synthesis of a novel glucose responsive and in-vitro pH triggered insulin delivery system comprised of glucose oxidase immobilized on acryloyl crosslinked dextran dialdehyde (ACDD) nanoparticles. Scanning electron microscopy, transmission electron microscopy and particle size analysis data revealed that these carriers possess nanosize which is an important parameter for drug delivery applications. In-vitro insulin release studies were performed under artificial gastric fluid (AGF, pH 1.2) and artificial intestinal fluid conditions (AIF, pH 7.4) at physiological temperature (37 °C). Insulin release profile showed controlled release of about 70% under AIF conditions for 24 h. Insulin release mechanism studied using different kinetic models revealed that Korsmeyer-Peppas model appropriately explained the mechanism as ʻnon-Fickian’ diffusion release of insulin. These glucose responsive stimuli sensitive nanocarriers exhibited controlled release of about 90% under AIF conditions in the presence of glucose. These findings revealed that these nanoparticles are promising and reliable delivery systems to overcome problems related with subcutaneous insulin therapy. |
| Databáze: | OpenAIRE |
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