A novel polypeptide from Meretrix meretrix Linnaeus inhibits the growth of human lung adenocarcinoma
| Autor: | Ming Liu, Linyou Cheng, Jianteng Wei, Ning Wu, Xiukun Lin, Lanhong Zheng, Cuicui Wang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Lung Neoplasms
Mice Nude Adenocarcinoma of Lung Antineoplastic Agents Apoptosis Adenocarcinoma Biology General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Western blot In vivo Carcinoma Non-Small-Cell Lung Cell Line Tumor medicine Animals Humans medicine.diagnostic_test Phosphatidylserine medicine.disease Xenograft Model Antitumor Assays Molecular biology chemistry Cell culture Premature chromosome condensation Immunology DNA fragmentation Peptides |
| Zdroj: | Experimental Biology and Medicine. 237:442-450 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1258/ebm.2012.011337 |
| Popis: | A novel polypeptide (Mere15) was purified from Meretrix meretrix Linnaeus by ammonium sulfate fractionation, ion exchange, gel filtration and reversed phase chromatography. Mere15 exhibited selective cytotoxicity to several human cancer cells. In vivo study showed that Mere15 significantly suppressed the growth of human lung adenocarcinoma A549 xenograft in nude mice. The mechanism was associated with a G2/M phase arrest followed by apoptosis, including membrane blebbing, loss of mitochondrial membrane potential, externalization of phosphatidylserine, chromosome condensation and DNA fragmentation. Western blot analysis showed that the intrinsic pathway was involved in Mere15-induced apoptosis. These results suggest that Mere15 may have therapeutic potential for the treatment of non-small-cell lung carcinoma. |
| Databáze: | OpenAIRE |
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