Alpha-2-macroglobulin and albumin are useful serum proteins to detect subclinical peritonitis in the rat
| Autor: | Wytske Westra, Cindy A M Dragt, Eelco D. Keuning, Jacob van den Born, Roos van Westrhenen, Johan K. Hiralall, Raymond T. Krediet, Liesbeth H P Hekking |
|---|---|
| Přispěvatelé: | Gastroenterology and Hepatology, Amsterdam Cardiovascular Sciences, Nephrology, Amsterdam Gastroenterology Endocrinology Metabolism, Tytgat Institute for Liver and Intestinal Research, Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Pathology Peritonitis/blood Peritoneal Dialysis/adverse effects medicine.medical_treatment 030232 urology & nephrology Serum albumin Peritonitis Enzyme-Linked Immunosorbent Assay Severity of Illness Index Gastroenterology Asymptomatic Peritoneal dialysis Serum Albumin/metabolism 03 medical and health sciences 0302 clinical medicine Internal medicine White blood cell medicine Animals Dialysis Solutions/toxicity alpha-Macroglobulins/metabolism 030212 general & internal medicine Rats Wistar biology business.industry Albumin General Medicine medicine.disease Blood proteins Rats Disease Models Animal medicine.anatomical_structure Nephrology biology.protein medicine.symptom business Biomarkers/blood Kidney disease |
| Zdroj: | Peritoneal dialysis international, 26(1), 101-107. Multimed Inc. Peritoneal dialysis international, 26(1), 101-107. MULTIMED INC |
| ISSN: | 0896-8608 |
| Popis: | BackgroundIn experimental peritoneal dialysis (PD) studies, the occurrence of peritonitis is a confounder in the interpretation of effects of chronic peritoneal exposure to dialysis solutions. Since fluid cannot be drained in most experimental PD models in the rat, it is impossible to diagnose peritonitis based on dialysate white blood cell counts. To study the value of serum markers for the presence of peritonitis, alpha-2-macroglobulin (α2M) and albumin were measured in rats with and without peritonitis after chronic exposure to dialysis solutions. To further investigate the time course of these markers in relation to the severity of peritonitis, nondialyzed rats were challenged with increasing numbers of bacteria and followed for 28 days.MethodsIn the first study, α2M and albumin were measured in rats exposed to glucose/lactate-based dialysis fluid before sacrifice. A comparison was made between animals with peritonitis, as judged from the presence of extensive infiltrates after sacrifice (gold standard) and/or clinical signs of peritonitis, or absence of peritonitis and infiltrates. In the second study, rats were intraperitoneally (IP) injected with 3 different concentrations of Staphylococcus aureus, and serum α2M and albumin were measured at various time points.ResultsIn the first study, serum α2M was higher and serum albumin was lower in animals with peritonitis compared to animals without peritonitis (both p < 0.05). In the second study, induction of α2M was clearly dependent on the inoculum concentration. Peak values of α2M were found at days 1 and 3. At all time points after inoculation, α2M was higher in all injected groups compared to the control group. Serum albumin values decreased in the highest inoculum group and remained decreased until 28 days after IP injection. Despite a low sensitivity, serum α2M >40 mg/L and albumin ConclusionsMeasurement of α2M and albumin once per month is an additional tool in the diagnosis of silent peritonitis in the chronic peritoneal exposure model in the rat. Levels of α2M >40 mg/L and albumin |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|